Carneiro Deisy G, Vidigal Pedro Marcus P, Morgan Túlio, Vanetti Maria Cristina D
Departamento de Microbiologia, Universidade Federal de Viçosa (UFV), Av. Peter Henry Rolfs, Viçosa, 36570-900, Minas Gerais, Brazil.
Núcleo de Análise de Biomoléculas (NuBioMol), Universidade Federal de Viçosa (UFV), Av. Peter Henry Rolfs, Viçosa 36570-900, Minas Gerais, Brazil.
Access Microbiol. 2024 Nov 4;6(11). doi: 10.1099/acmi.0.000828.v3. eCollection 2024.
serotype Enteritidis is a generalist serotype that adapts to different hosts and transmission niches. It has significant epidemiological relevance and is among the most prevalent serotypes distributed in several countries. Enteritidis causes self-limited gastroenteritis in humans, which can progress to systemic infection in immunocompromised individuals. The pathogenicity mechanism is multifactorial and complex, including the presence of virulence factors that are encoded by virulence genes. Poultry products are considered significant reservoirs of many serotypes, and Enteritidis infections are often related to the consumption of chicken meat and eggs. This study reports the whole-genome sequence of Enteritidis PT4 strain 578. A total of 165 genes (3.66%) of the 4506 coding sequences (CDS) predicted in its genome are virulence factors associated with cell invasion, intestinal colonization, and intracellular survival. The genome harbours twelve pathogenicity islands (SPIs), with the SPI-1 and SPI-2 genes encoding type III secretion systems (T3SS) showing high conservation. Six prophage-related sequences were found, with regions of intact prophages corresponding to and . The genome also contains two CRISPR systems. Comparative genome analysis with Enteritidis ATCC 13076, Typhimurium ATCC 13311, and Typhimurium ATCC 14028 demonstrates that most unshared genes are related to metabolism, membrane, and hypothetical proteins. Finally, the phenotypic characterization evidenced differences among Enteritidis PT4 578 and the other three serotypes regarding the expression of the red, dry, and rough (rdar) morphotype and biofilm formation. Overall, the genomic characterization and phenotypic properties expand knowledge of the mechanisms of pathogenicity in Enteritidis PT4 578.
肠炎血清型是一种能适应不同宿主和传播生态位的通用血清型。它具有重要的流行病学意义，是在多个国家分布最为普遍的血清型之一。肠炎血清型可导致人类自限性肠胃炎，在免疫功能低下的个体中可能发展为全身感染。其致病机制是多因素且复杂的，包括由毒力基因编码的毒力因子的存在。家禽产品被认为是许多血清型的重要储存宿主，肠炎血清型感染通常与鸡肉和鸡蛋的消费有关。本研究报告了肠炎血清型PT4菌株578的全基因组序列。在其基因组中预测的4506个编码序列（CDS）中，共有165个基因（3.66%）是与细胞侵袭、肠道定植和细胞内存活相关的毒力因子。该基因组含有12个致病岛（SPIs），其中编码III型分泌系统（T3SS）的SPI-1和SPI-2基因具有高度保守性。发现了6个与前噬菌体相关的序列，完整前噬菌体区域分别对应于 和 。该基因组还包含两个CRISPR系统。与肠炎血清型ATCC 13076、鼠伤寒血清型ATCC 13311和鼠伤寒血清型ATCC 14028的比较基因组分析表明，大多数非共享基因与代谢、膜和假定蛋白有关。最后，表型特征证明了肠炎血清型PT4 578与其他三种血清型在红色、干燥和粗糙（rdar）形态型表达和生物膜形成方面存在差异。总体而言，基因组特征和表型特性扩展了对肠炎血清型PT4 578致病机制的认识。
