[前S1蛋白、乙肝病毒脱氧核糖核酸及乙肝e抗原在慢性乙型肝炎患者病毒复制诊断中的对比研究]

[The contrast study of Pre-S1 protein, HBV-DNA and HBeAg in diagnosing viral replication in patients with chronic hepatitis B].

作者信息

Li Qin, Sun Gui-zhen, Wei Yu-xiang, Min Yu-yuan, Cong Yu-long, Ou Xiao-juan, Wang Bao-en

机构信息

Liver Research Center, Beijing Friendship Hospital Affiliate of Capital University of Medical Sciences, Beijing 100050, China.

出版信息

Zhonghua Gan Zang Bing Za Zhi. 2004 Mar;12(3):134-6.

PMID:15059293

Abstract

OBJECTIVE

To determine the role of Pre-S1 protein in diagnosing viral replication in patients with chronic hepatitis B.

METHODS

104 consecutive patients with chronic hepatitis B were included in the study, liver biopsy were performed in all patients. Serial serum samples were studied with the quantitative determination of HBV-DNA by a quantitative PCR assay, determination of Pre-S1 protein by ELISA.

RESULTS

The positive rates of HBV-DNA and Pre-S1 protein in patients with HBsAg HBeAg anti-HBc (+) both were 96.5%. The positive rates of HBV-DNA and Pre-S1 protein in patients with HBsAg anti-HBe anti-HBc (+) were 81.5%, 72.3%, respectively. The positive rates of HBV-DNA and Pre-S1 protein in patients with HBsAg anti-HBc (+) were 87.5%, 75.0%, respectively. It represented some patients with HBeAg (-) anti-HBe (+/-) still had viral replication. HBV-DNA>10(3) copy/ml as positive criteria for diagnosing viral replication, the positive rate of HBeAg, Pre-S1 were 31.5% (28/89), 80.9% (72/89) in patients with HBV-DNA>10(3) copy/ml, respectively. The concordance rates of HBeAg, Pre-S1 with HBV-DNA were 40.0% (42/104), 82.0% (85/104), respectively.

CONCLUSION

It showed that Pre-S1 was more sensitive than HBeAg in diagnosing viral replication in patients with chronic hepatitis B.

摘要

目的

确定前S1蛋白在慢性乙型肝炎患者病毒复制诊断中的作用。

方法

本研究纳入104例连续的慢性乙型肝炎患者，所有患者均进行了肝活检。采用定量聚合酶链反应法对系列血清样本进行HBV-DNA定量检测，采用酶联免疫吸附测定法检测前S1蛋白。

结果

HBsAg、HBeAg、抗-HBc（+）患者的HBV-DNA和前S1蛋白阳性率均为96.5%。HBsAg、抗-HBe、抗-HBc（+）患者的HBV-DNA和前S1蛋白阳性率分别为81.5%、72.3%。HBsAg、抗-HBc（+）患者的HBV-DNA和前S1蛋白阳性率分别为87.5%、75.0%。这表明部分HBeAg（-）、抗-HBe（+/-）患者仍有病毒复制。以HBV-DNA>10³拷贝/ml作为诊断病毒复制阳性标准，HBV-DNA>10³拷贝/ml患者中HBeAg、前S1蛋白阳性率分别为31.5%（28/89）、80.9%（72/89）。HBeAg、前S1蛋白与HBV-DNA的符合率分别为40.0%（42/104）、82.0%（85/104）。