Xing Guo-hong, Zhang Jin-chuan
Respiratory Department of Nanlou, PLA General Hospital, Beijing 100853, China.
Zhonghua Zhong Liu Za Zhi. 2004 Jan;26(1):36-9.
To screen genes with abnormal expression in poorly-differentiated human lung adenocarcinoma at stage I with cDNA chip.
The mRNA was extracted from cancer tissue and matched normal lung tissue, and was labeled by Cy5-dUTP or Cy3-dUTP as probes. Subsequently, the mixed probes were hybridized to the cDNA chip containing 8192 genes. The information was obtained by managing the cDNA chip with ScanArray4000 scanner and GenePix3.0 software.
Five hundred and eighty genes were differentially expressed between cancer and normal lung tissue. Compared with normal lung tissue, 405 genes were up-regulated and 175 genes were down-regulated in cancer tissue. These genes are involved in different cell activities such as growth regulation and signal transduction. Among the 66 genes with remarkable differential expression between the two tissues, 39 were up-regulated and 27 down-regulated.
Many different kinds of genes are possibly involved in the initiation and progression of human lung adenocarcinoma. cDNA chip technique might be a useful method in screening lung cancer implicated genes.
利用cDNA芯片筛选Ⅰ期低分化人肺腺癌中表达异常的基因。
从癌组织及配对的正常肺组织中提取mRNA，用Cy5-dUTP或Cy3-dUTP标记作为探针。随后，将混合探针与包含8192个基因的cDNA芯片杂交。通过ScanArray4000扫描仪和GenePix3.0软件处理cDNA芯片获取信息。
癌组织与正常肺组织之间有580个基因表达差异。与正常肺组织相比，癌组织中有405个基因上调，175个基因下调。这些基因参与不同的细胞活动，如生长调节和信号转导。在两种组织间表达差异显著的66个基因中，39个上调，27个下调。
多种不同基因可能参与人肺腺癌的发生和发展。cDNA芯片技术可能是筛选肺癌相关基因的一种有用方法。
