Xu Bao-yan, Wang Yu-ming, Deng Guo-hong, Huang Yan-ping, Zhong Lü-hua, Liu Guo-dong, Tan Zhao-xia, Fan Yi, Ding Shi-tao
Institute of Infectious Diseases of PLA, Southwest Hospital, Third Military Medical University, Chongqing 400038, China.
Zhonghua Yi Xue Za Zhi. 2004 Feb 2;84(3):189-93.
The primary comparative analysis between the host genetic factors and their relationships with clinical phenotype of 20 pairs of HBV infected and high risk twins.
Zygosity of twins was diagnosed by STR microsatellite polymorphism analysis. To identify the serological model and exclude the evidence of coinfection with other virus, we detected HAV, HBV, HCV, HDV, HEV serological markers by electrochemiluminescence method. HBV DNA level was detected by Lightcycler Fluorescent Quantitative system and Liver function (ALT, AST, TBil) was detected by HITACHI7250 Biochemistry Detection System. The data was analysis by Fisher's exact test to comparatively analyze among the monozygotic twins (MZ), dizygotic twins (DZ) and control groups.
The significant difference was found in the concordance rate of disease, concordance of clinical phenotype and serological patterns of HBV infection between the MZ and DZ twins (P < 0.05), it was also found between MZ and control groups (P < 0.05), but not between DZ and control groups (P > 0.05). No significant difference in the concordance of other HBV infection markers was observed (P > 0.05). Concordance of clinical phenotype may be related to patients' age and antivirus therapy. Injection with the HBIg and initiative vaccination right after birth can prevent twins with high risk to infection to be victims.
The significant difference was found in the concordance rate, concordance of clinical phenotype and serological patterns between MZ and control groups, it was also found between MZ and DZ groups, which is correspond to the opinion of the high concordance of MZ and indicated the host genetic factors may play role in influencing the clinical phenotype, while other factors such as the vaccination may have an effect on the clinical phenotype in some extent.
对20对乙肝病毒感染高危双胞胎的宿主遗传因素及其与临床表型的关系进行初步比较分析。
采用STR微卫星多态性分析诊断双胞胎的合子类型。为确定血清学模式并排除合并感染其他病毒的证据，我们采用电化学发光法检测甲型肝炎病毒、乙肝病毒、丙型肝炎病毒、丁型肝炎病毒、戊型肝炎病毒的血清学标志物。采用Lightcycler荧光定量系统检测乙肝病毒DNA水平，采用日立7250生化检测系统检测肝功能（谷丙转氨酶、谷草转氨酶、总胆红素）。采用Fisher精确检验对数据进行分析，以比较单卵双胞胎（MZ）、双卵双胞胎（DZ）和对照组之间的差异。
MZ和DZ双胞胎在疾病一致率、临床表型一致性和乙肝病毒感染血清学模式方面存在显著差异（P<0.05），MZ与对照组之间也存在显著差异（P<0.05），但DZ与对照组之间无显著差异（P>0.05）。其他乙肝病毒感染标志物的一致性未观察到显著差异（P>0.05）。临床表型的一致性可能与患者年龄和抗病毒治疗有关。出生后立即注射乙肝免疫球蛋白和主动接种疫苗可预防高危感染双胞胎发病。
MZ与对照组在一致率、临床表型一致性和血清学模式方面存在显著差异，MZ与DZ组之间也存在显著差异，这与MZ高度一致的观点相符，表明宿主遗传因素可能在影响临床表型方面发挥作用，而其他因素如疫苗接种在一定程度上可能对临床表型产生影响。
