工程化沙门氏菌鞭毛融合蛋白FliR-FlhB的分析
Analysis of an engineered Salmonella flagellar fusion protein, FliR-FlhB.
作者信息
Van Arnam John S, McMurry Jonathan L, Kihara May, Macnab Robert M
机构信息
Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520, USA.
出版信息
J Bacteriol. 2004 Apr;186(8):2495-8. doi: 10.1128/JB.186.8.2495-2498.2004.
Abstract
Salmonella FliR and FlhB are membrane proteins necessary for flagellar export. In Clostridium a fliR-flhB fusion gene exists. We constructed a similar Salmonella fusion gene which is able to complement fliR, flhB, and fliR flhB null strains. Western blotting revealed that the FliR-FlhB fusion protein retains the FlhB protein's cleavage properties. We conclude that the FliR and FlhB proteins are physically associated in the wild-type Salmonella basal body, probably in a 1:1 ratio.
摘要
鼠伤寒沙门氏菌的FliR和FlhB是鞭毛输出所必需的膜蛋白。在梭菌中存在一个fliR - flhB融合基因。我们构建了一个类似的沙门氏菌融合基因，它能够互补fliR、flhB和fliR flhB缺失菌株。蛋白质免疫印迹分析表明，FliR - FlhB融合蛋白保留了FlhB蛋白的切割特性。我们得出结论，在野生型沙门氏菌基体中，FliR和FlhB蛋白在物理上相互关联，可能以1:1的比例存在。
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