Poloumienko Arkadi
Department of Human Biology and Nutritional Sciences, University of Guelph, Guelph, Canada.
Genome. 2004 Feb;47(1):74-83. doi: 10.1139/g03-099.
A growing body of evidence suggests the involvement of sex chromosome genes in mammalian development. We report the cloning and characterization of the complete coding regions of the bovine Y chromosome ZFY and X chromosome ZFX genes, and partial coding regions of porcine and equine ZFX and ZFY genes. Bovine ZFY and ZFX are highly similar to each other and to ZFX and ZFY from other species. While bovine and human ZFY proteins are both 801 amino acids long, bovine ZFX is 5 amino acids shorter than human ZFX. Like in humans, both bovine ZFY and ZFX contain 13 zinc finger motifs and belong to the Krueppel family of C2H2-type zinc finger proteins. The internal exon-intron organization of the bovine, porcine and equine ZFX and ZFY genes has been determined and compared. Within this region, the exon lengths and the positions of the splice sites are conserved, further suggesting a high evolutionary conservation of the ZFX and ZFY genes. Additionally, new alternatively spliced forms of human ZFX have been identified.
越来越多的证据表明性染色体基因参与哺乳动物的发育。我们报告了牛Y染色体ZFY和X染色体ZFX基因完整编码区的克隆与表征，以及猪和马ZFX和ZFY基因部分编码区的情况。牛的ZFY和ZFX彼此高度相似，也与其他物种的ZFX和ZFY高度相似。牛和人类的ZFY蛋白均长801个氨基酸，而牛的ZFX比人类的ZFX短5个氨基酸。与人类一样，牛的ZFY和ZFX都含有13个锌指基序，属于C2H2型锌指蛋白的Krueppel家族。已确定并比较了牛、猪和马ZFX和ZFY基因的内部外显子 - 内含子组织。在该区域内，外显子长度和剪接位点的位置是保守的，这进一步表明ZFX和ZFY基因具有高度的进化保守性。此外，还鉴定出了人类ZFX新的可变剪接形式。
