用于多噬棘阿米巴诊断和鉴定的特异性引物对的设计与评估
Design and evaluation of a specific primer pair for the diagnosis and identification of Acanthamoeba polyphaga.
作者信息
Ortega-Rivas Antonio, Lorenzo-Morales Jacob, Martinez-Carretero Enrique, Villa Lloberas Mercedes, Valladares Hernández Basilio, del Castillo-Remiro Antonio
机构信息
Department of Parasitology, Faculty of Pharmacy, University of La Laguna, Avda. Francisco Sánchez s/n, C.P. 38271, La Laguna, Tenerife, Spain.
出版信息
Curr Microbiol. 2004 May;48(5):360-3. doi: 10.1007/s00284-003-4211-5.
Random amplified polymorphism DNA (RAPD) is a useful tool for species identification. The obtained band patterns can be used for specific primer pair design that may be useful for species diagnosis. In this study, a distinctive a 962-bp band in A. polyphaga band patterns was found, by using the OPC20 primer (ACTTCGCCAC). The DNA fragment was used to design a specific primer pair that was useful for the identification of different isolates as A. polyphaga species. A case of A. polyphaga in disseminated acanthamoebiasis affecting mesenteric nodes is also reported.
随机扩增多态性DNA（RAPD）是一种用于物种鉴定的有用工具。所获得的条带模式可用于设计特异性引物对，这可能对物种诊断有用。在本研究中，通过使用OPC20引物（ACTTCGCCAC），在多食棘阿米巴的条带模式中发现了一条独特的962bp条带。该DNA片段被用于设计一对特异性引物对，可用于鉴定不同分离株是否为多食棘阿米巴物种。本文还报告了一例播散性棘阿米巴病累及肠系膜淋巴结的多食棘阿米巴病例。
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