Hume Michael E, Poole Toni L, Pultz Nicole J, Hanrahan Jennifer A, Donskey Curtis J
United States Department of Agriculture, Agricultural Research Service, Southern Plains Agricultural Research Center, Food and Feed Safety Research Unit, College Station, TX 77845, USA.
Curr Microbiol. 2004 May;48(5):364-7. doi: 10.1007/s00284-003-4112-7.
A continuous-flow competitive exclusion (CFCE) culture model of human stool microflora was used to examine whether supplemental anaerobic gas is necessary for maintenance of anaerobes and inhibition of vancomycin-resistant Enterococcus (VRE). CFCE cultures of human stool microflora were maintained with supplemental nitrogen, without supplemental nitrogen, or with percolated room air. Cultures with or without supplemental nitrogen maintained >9 log(10) CFU mL(-1) of obligate anaerobes and eliminated 10(6) CFU mL(-1) of VRE. When room air was percolated into the culture, anaerobes were detected at < or =2 log(10) CFU mL(-1), and the same VRE inoculum was not eliminated ( P < 0.001). These data demonstrate that human stool CFCE cultures maintain high levels of obligate anaerobes and inhibit VRE without the addition of supplemental anaerobic gas.
采用人粪便微生物群的连续流竞争排除（CFCE）培养模型，以检验补充厌氧气体对于维持厌氧菌及抑制耐万古霉素肠球菌（VRE）是否必要。人粪便微生物群的CFCE培养物分别在补充氮气、不补充氮气或通入室内空气的条件下维持培养。补充或不补充氮气的培养物中，专性厌氧菌的含量维持在>9 log(10) CFU mL(-1)，且能清除10(6) CFU mL(-1)的VRE。当向培养物中通入室内空气时，检测到厌氧菌含量≤2 log(10) CFU mL(-1)，且相同的VRE接种物未被清除（P < 0.001）。这些数据表明，人粪便CFCE培养物在不添加补充厌氧气体的情况下，仍能维持高水平的专性厌氧菌并抑制VRE。
