Al-Mufti R, Hambley H, Farzaneh F, Nicolaides K H
Harris Birthright Research Centre For Fetal Medicine, King's College London School of Medicine, King's College Hospital, Denmark Hill, London, UK.
J Matern Fetal Neonatal Med. 2003 Dec;14(6):392-7. doi: 10.1080/14767050412331312240.
To determine the distribution of fetal erythroblasts in the maternal circulation at different gestations.
Maternal blood was obtained from 152 normal singleton pregnancies at 11-40 weeks of gestation. Fetal erythroblasts were isolated using triple density gradient separation and anti-CD71 magnetic cell sorting techniques. The enriched erythroblasts were stained with Kleihauer-Giemsa and with fluorescent antibodies for the zeta (zeta), epsilon (epsilon) and gamma (gamma) globin chains. The percentage of fetal cells positive for each stain was calculated. Fluorescence in situ hybridization for X and Y chromosomes was also performed. Comparison was made in the proportion of positive fetal erythroblasts among the different gestational ages.
The proportion of erythroblasts stained positive with gamma-globin chain and Kleihauer-Giemsa decreased with gestation from a median of 2% at 11 weeks to 0.5% at 40 weeks. Similarly, there was a decrease in the percentage of Y-signal-positive cells from 1% at 11 weeks to 0.3% at 40 weeks. The proportion of enriched fetal erythroblasts stained positive with zeta- and epsilon-globin chains decreased exponentially from respective medians of 0.6% and 1.5% at 11 weeks to zero after 19 weeks and 24 weeks.
In normal singleton pregnancy the percentage of fetal erythroblasts enriched from maternal blood decreases with gestation.
确定不同孕周时母血中胎儿成红细胞的分布情况。
从152例孕11 - 40周的正常单胎妊娠孕妇采集母血。采用三重密度梯度分离法和抗CD71磁性细胞分选技术分离胎儿成红细胞。对富集的成红细胞进行Kleihauer - Giemsa染色以及用荧光抗体检测ζ、ε和γ珠蛋白链。计算每种染色阳性的胎儿细胞百分比。还进行了X和Y染色体的荧光原位杂交。比较不同孕周中阳性胎儿成红细胞的比例。
γ珠蛋白链和Kleihauer - Giemsa染色阳性的成红细胞比例随孕周增加而降低，从孕11周时的中位数2%降至孕40周时的0.5%。同样，Y信号阳性细胞百分比从孕11周时的1%降至孕40周时的0.3%。ζ和ε珠蛋白链染色阳性的富集胎儿成红细胞比例分别从孕11周时的中位数0.6%和1.5%呈指数下降，在孕19周和24周后降至零。
在正常单胎妊娠中，从母血中富集的胎儿成红细胞百分比随孕周增加而降低。
