Angus R B, Kemeny C K, Wood E L
School of Biological Sciences, Royal Holloway, University of London, Egham, Surrey, UK.
Hereditas. 2004;140(2):134-8. doi: 10.1111/j.1601-5223.2004.01815.x.
Chromosome preparations were made from mid-gut and ovarian cells of adult Notonecta glauca L., N. obliqua Thunberg, N. maculata F. and N. viridis Delcourt, using the acetic acid dissociation, air-drying method (Crozier 1968) with Giemsa staining. C-banding was obtained by treatment with barium hydroxide and salt-sodium citrate (2xSSC). The karyotypes of the first three species are very similar, with 11 pairs of autosomes plus XY sex chromosomes (plus sometimes a small 12th autosome pair in N. glauca), and the sequence of chromosome sizes very similar. However, the four longest pairs of autosomes, and the X chromosomes, have characteristic C-band patterns, which differ between the species. The karyotype of N. viridis is more distinct, with one pair of long autosomes, while the remaining chromosomes are much shorter. The long autosomes have distinct C-bands, but these are present in only one of the shorter pairs, as faint terminal bands. In warm conditions the long autosomes of N. viridis appear rod-like, but in cold conditions they have one end heavily condensed, giving a tadpole-like appearance.
采用醋酸解离、空气干燥法（Crozier，1968）并进行吉姆萨染色，从成年黄仰蝽（Notonecta glauca L.）、斜纹仰蝽（N. obliqua Thunberg）、黄斑仰蝽（N. maculata F.）和绿仰蝽（N. viridis Delcourt）的中肠和卵巢细胞制备染色体标本。通过用氢氧化钡和盐 - 柠檬酸钠（2×SSC）处理获得C带。前三个物种的核型非常相似，有11对常染色体加XY性染色体（黄仰蝽有时还会有一对小的第12对常染色体），染色体大小顺序非常相似。然而，四对最长的常染色体和X染色体具有特征性的C带模式，不同物种之间存在差异。绿仰蝽的核型更为独特，有一对长常染色体，而其余染色体则短得多。长常染色体有明显的C带，但仅在较短的一对染色体中有微弱的末端带。在温暖条件下，绿仰蝽的长常染色体呈棒状，但在寒冷条件下，其一端高度浓缩，呈现出蝌蚪状外观。
