Murray G I
Department of Pathology, University of Aberdeen, UK.
Histochem J. 1992 Jul;24(7):399-408. doi: 10.1007/BF01089102.
Freeze-drying or freeze-substitution, combined with low-temperature resin-embedding, represents a new approach to the optimum preservation of tissue for enzyme histochemistry and immunohistochemistry. This method, which avoids tissue fixation, combines excellent tissue morphology with the preservation of enzyme activity and immunoreactivity and allows high-resolution enzyme histochemical and immunohistochemical studies to be performed. The activity of a wide range of enzymes can be demonstrated in sections of freeze-dried or freeze-substituted resin-embedded tissue. Enzymes are retained in situ with high activity, accurate localization and no diffusion. Immunohistochemical studies can also be performed on resin sections, and antigens--especially labile antigens--are immobilized in situ without denaturation and can be demonstrated with high sensitivity and accurately localized. This method allows the localization and distribution of enzymes and antigens to be studied in relation to excellent histological and cytological detail.
冷冻干燥或冷冻置换，结合低温树脂包埋，是一种用于酶组织化学和免疫组织化学的组织最佳保存的新方法。这种方法避免了组织固定，将出色的组织形态与酶活性和免疫反应性的保存相结合，并允许进行高分辨率的酶组织化学和免疫组织化学研究。在冷冻干燥或冷冻置换树脂包埋组织的切片中，可以显示多种酶的活性。酶以高活性、准确定位且无扩散的方式保留在原位。免疫组织化学研究也可以在树脂切片上进行，抗原——尤其是不稳定抗原——在原位固定而不发生变性，并且可以高灵敏度地显示并准确定位。这种方法能够在出色的组织学和细胞学细节背景下研究酶和抗原的定位与分布。
