Fortier I, Gallant M A, Hackett J A, Patry C, de Brum-Fernandes A J
Rheumatic Diseases Unit, Department of Medicine, Faculté de Médecine, Centre Hospitalier Université de Sherbrooke, Centre de Recherches Clinques, 3001 12th Avenue Nord, Fleurimont, Québec, Sherbrooke, Canada PQ J1H 5N4.
Prostaglandins Leukot Essent Fatty Acids. 2004 May;70(5):431-9. doi: 10.1016/j.plefa.2003.08.024.
PGE(2) is an important mediator of bone metabolism, but the precise localization of its receptors in human bone remains unknown. The present study used specific antibodies against EP(1), EP(2), EP(3) and EP(4) receptors for immunolocalization in normal, osteoporotic and pagetic human adult bone and in human foetal bone. No labelling was obtained for the EP(1) and EP(2) receptors. The EP(3) receptor was detected in foetal osteoclasts, osteoblasts and osteocytes, but only in osteoclasts and some osteoblasts from adult bone. The EP(4) receptor was detected in foetal osteoclasts, osteoblasts and osteocytes and in adult osteoclasts and osteoblasts, but not in adult osteocytes. Our results show differences in PGE(2) receptor expression in foetal and adult human bone but no difference in adult normal compared to pathologic bone. Finally, these results show that the distribution of EP receptors in human osteoblasts in bone corresponds in part to what we recently described in human osteoblasts in culture.
前列腺素E2（PGE(2)）是骨代谢的重要介质，但其受体在人体骨骼中的精确定位尚不清楚。本研究使用针对EP(1)、EP(2)、EP(3)和EP(4)受体的特异性抗体，对正常、骨质疏松和畸形性骨炎的成人骨以及胎儿骨进行免疫定位。未检测到EP(1)和EP(2)受体的标记。EP(3)受体在胎儿破骨细胞、成骨细胞和骨细胞中被检测到，但仅在成人骨的破骨细胞和一些成骨细胞中被检测到。EP(4)受体在胎儿破骨细胞、成骨细胞和骨细胞以及成人破骨细胞和成骨细胞中被检测到，但在成人骨细胞中未被检测到。我们的结果显示，胎儿和成人骨中PGE(2)受体表达存在差异，但与病理骨相比，成人正常骨中无差异。最后，这些结果表明，EP受体在人体骨中成骨细胞中的分布部分与我们最近在培养的人体成骨细胞中描述的情况一致。
