一种新型十八烷神经肽（ODN）拮抗剂对雄性大鼠大脑中促性腺激素释放激素基因表达的体内作用。

In vivo action of a new octadecaneuropeptide (ODN) antagonist on gonadotropin-releasing hormone gene expression in the male rat brain.

作者信息

Compère V, Li S, Leprince J, Tonon M C, Vaudry H, Pelletier G

机构信息

European Institute for Peptide Research (IFRMP 23), Laboratory of Cellular and Molecular Neuroendocrinology, INSERM U413, UA CNRS, University of Rouen, Mont-Saint-Aignan, France.

出版信息

Neuroscience. 2004;125(2):411-5. doi: 10.1016/j.neuroscience.2004.02.016.

DOI:10.1016/j.neuroscience.2004.02.016

PMID:15062983

Abstract

It has been reported that several of the effects induced by octadecaneuropeptide (ODN) could be mediated by an activation of a metabotropic receptor. In order to investigate the role and mechanism of action of ODN in gonadotropin-releasing hormone (GnRH) neuron regulation, we studied the effects of the acute i.c.v. administration of ODN and of a new ODN antagonist to metabotropic receptor, cyclo(1-8)[Dleu(5)]OP, on GnRH mRNA expression as evaluated by in situ hybridization in castrated male rats. The administration of ODN produced a decrease in the hybridization signal while the administration of cyclo(1-8)[Dleu(5)]OP alone produced an 18% increase. When administrated concomitantly with ODN, the antagonist both inhibited the depressing effect of ODN and induced a 22% increase over the values detected in ODN-treated rats. The data suggest that the effect of ODN on GnRH mRNA expression might be mediated by interaction with metabotropic receptors.

摘要

据报道，十八烷神经肽（ODN）诱导的几种效应可能由代谢型受体的激活介导。为了研究ODN在促性腺激素释放激素（GnRH）神经元调节中的作用和作用机制，我们通过原位杂交评估了去势雄性大鼠中急性脑室内注射ODN和一种新的ODN代谢型受体拮抗剂环（1-8）[Dleu（5）]OP对GnRH mRNA表达的影响。ODN给药导致杂交信号降低，而单独给予环（1-8）[Dleu（5）]OP则使杂交信号增加18%。当与ODN同时给药时，该拮抗剂既抑制了ODN的抑制作用，又使检测值比ODN处理的大鼠增加了22%。数据表明，ODN对GnRH mRNA表达的影响可能通过与代谢型受体的相互作用介导。