βγ亚基介导神经肽Y对三磷酸腺苷刺激的肌醇磷酸生成的增强作用。
Betagamma subunits mediate the NPY enhancement of ATP-stimulated inositol phosphate formation.
作者信息
Li Xinying, Ikezu Tsuneya, Hexum Terry D
机构信息
Department of Pharmacology, 986260 Nebraska Medical Center, University of Nebraska Medical Center, Omaha, NE 68198-6260, USA.
出版信息
Peptides. 2004 Feb;25(2):267-74. doi: 10.1016/j.peptides.2003.12.019.
Neuropeptide Y (NPY) enhances ATP-stimulated inositol phosphate (InsP) formation in bovine chromaffin cells through an unknown mechanism. Chromaffin cells were transduced with the carboxyl terminus of beta-adrenergic receptor kinase 1 (betaARK1CT), a Gbetagamma subunits scavenger, using a recombinant adenovirus system. The adenovirus also expresses a green fluorescent protein (GFP) which serves as an index of transduction. Flow cytometry showed that up to 80% of chromaffin cells were transduced by the virus. There was a direct correlation between the betaARK1CT inhibition of the NPY enhancement of ATP-stimulated InsP formation and the percent of cells expressing GFP ( r2=0.9993 ). These results demonstrate that Gbetagamma subunits are required for the NPY enhancement of ATP-stimulated InsP formation in bovine chromaffin cells.
神经肽Y（NPY）通过未知机制增强牛嗜铬细胞中ATP刺激的肌醇磷酸（InsP）生成。使用重组腺病毒系统，用β-肾上腺素能受体激酶1（βARK1CT）的羧基末端（一种Gβγ亚基清除剂）转导嗜铬细胞。该腺病毒还表达绿色荧光蛋白（GFP），用作转导指标。流式细胞术显示高达80%的嗜铬细胞被病毒转导。βARK1CT对NPY增强ATP刺激的InsP生成的抑制作用与表达GFP的细胞百分比之间存在直接相关性（r2 = 0.9993）。这些结果表明，Gβγ亚基是牛嗜铬细胞中NPY增强ATP刺激的InsP生成所必需的。
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