利用胸膜肺炎放线杆菌的自然转化：一种等位基因替换的简单方法。
Harnessing natural transformation in Actinobacillus pleuropneumoniae: a simple method for allelic replacements.
作者信息
Bossé Janine T, Nash John H E, Kroll J Simon, Langford Paul R
机构信息
Molecular Infectious Disease Group, Department of Paediatrics, Faculty of Medicine, Imperial College London, St. Mary's Campus, Norfolk Place, London W2 1PG, UK.
出版信息
FEMS Microbiol Lett. 2004 Apr 15;233(2):277-81. doi: 10.1016/j.femsle.2004.02.022.
We have investigated the use of a natural transformation protocol to introduce mutations into Actinobacillus pleuropneumoniae serotypes 1 and 5b. For both strains tested, we recovered 1 in 10(8) transformants during culture in rich medium, a result that was independent of the growth phase. This low frequency of transformation of A. pleuropneumoniae did not increase when bacteria were grown under conditions known to be optimal for induction of competence in Haemophilus influenzae. Using linearised plasmid DNA containing a kanamycin cassette inserted into the sodC gene of A. pleuropneumoniae serotype 1, we showed that natural transformation can be used as a simple method for introducing allele replacements into this bacterium, and can be used to transfer mutations from one serotype to another.
我们研究了使用自然转化方案将突变引入胸膜肺炎放线杆菌血清型1和5b的情况。对于所测试的两种菌株，我们在丰富培养基培养过程中每10⁸个转化体中回收1个，该结果与生长阶段无关。当细菌在已知对流感嗜血杆菌感受态诱导最适宜的条件下生长时，胸膜肺炎放线杆菌这种低转化频率并未增加。使用含有插入胸膜肺炎放线杆菌血清型1 sodC基因的卡那霉素盒的线性化质粒DNA，我们表明自然转化可作为一种将等位基因替换引入该细菌的简单方法，并且可用于将突变从一种血清型转移到另一种血清型。
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