Zamora Mònica, Granell Meritxell, Mampel Teresa, Viñas Octavi
Departament de Bioquímica i Biologia Molecular, Facultat de Biologia, Universitat de Barcelona, Diagonal 645, E-08028 Barcelona, Spain.
FEBS Lett. 2004 Apr 9;563(1-3):155-60. doi: 10.1016/S0014-5793(04)00293-5.
Mitochondrial adenine nucleotide translocase 1 (ANT1), but not ANT2, can dominantly induce apoptosis. Nothing is known, however, about the apoptotic activity of ANT3. We have transfected HeLa cells with the three human ANT isoforms to compare their potential as inducers of apoptosis. Transient overexpression of ANT3 resulted, like ANT1, in apoptosis as shown by an increase in the sub-G1 fraction, annexin V staining, low DeltaPsi(m), and activation of caspases 9 and 3. Moreover, the apoptosis produced by ANT3 was inhibited by bongkrekic acid and by cyclosporin A. The pro-apoptotic activities of the ANT1 and ANT3 isoforms contrast with the lack of apoptotic activity of ANT2. This finding may help to identify the specific factors associated with the pro-apoptotic activities of ANT isoforms.
线粒体腺嘌呤核苷酸转位酶1（ANT1）而非ANT2可显性诱导细胞凋亡。然而，关于ANT3的凋亡活性却一无所知。我们已将三种人类ANT异构体转染至HeLa细胞，以比较它们作为凋亡诱导剂的潜力。如ANT1一样，ANT3的瞬时过表达导致细胞凋亡，表现为亚G1期细胞比例增加、膜联蛋白V染色、线粒体膜电位降低以及半胱天冬酶9和3的激活。此外，ANT3诱导的细胞凋亡受到硼酸和环孢菌素A的抑制。ANT1和ANT3异构体的促凋亡活性与ANT2缺乏凋亡活性形成对比。这一发现可能有助于识别与ANT异构体促凋亡活性相关的特定因素。
