用于在体内检测蛋白质-蛋白质相互作用的哺乳动物双杂交检测法。

Mammalian two-hybrid assay for detecting protein-protein interactions in vivo.

作者信息

Lee Jae Woon, Lee Soo-Kyung

机构信息

Department of Medicine, Division of Diabetes, Endocrinology and Medicine, Baylor College of Medicine, 1 Baylor Plaza, Houston, TX, USA.

出版信息

Methods Mol Biol. 2004;261:327-36. doi: 10.1385/1-59259-762-9:327.

DOI:10.1385/1-59259-762-9:327

PMID:15064467

Abstract

Mammalian two-hybrid assay is a convenient, powerful tool to investigate protein-protein interactions in vivo. In particular, this method has a major advantage over the better known yeast version in that one can study interactions between mammalian proteins that may not fold correctly in yeast or that require post-translational modification or external stimulation that are not present in yeast.

摘要

哺乳动物双杂交分析是一种在体内研究蛋白质-蛋白质相互作用的便捷而强大的工具。特别是，该方法相对于更为知名的酵母双杂交方法具有一个主要优势，即可以研究那些在酵母中可能无法正确折叠、或者需要酵母中不存在的翻译后修饰或外部刺激的哺乳动物蛋白质之间的相互作用。