Yang Fen, Li Ying-hui, Nie Jie-lin
Institute of Space Medico-Engineering, Beijing, P. R. China 100094.
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2004 Mar;18(2):119-22.
To study three-dimensional culturing methods of neonatal rat cardiac myocytes in simulated microgravity.
Neonatal rat primary cardiac myocytes were separated and seeded into polylactic acid scaffolds, stirred in spinner flasks for 24 hours, and then moved into rotary cell culture system for three-dimensional culture. The growth of cardiac myocytes was observed under inverted phase contrast microscope, scanning electron microscope and transmission electron microscope, and metabolic assay was assessed by MTT assay.
Cardiac myocytes with sustained metabolic activity attached to the polylactic acid scaffolds, extended and confluenced. Pulsations of PLA-cardiac myocytes was found in some areas.
The rotary cell culture system is suitable to develop neonatal rat cardiac myocytes culturing for three-dimensional modeling.
研究新生大鼠心肌细胞在模拟微重力条件下的三维培养方法。
分离新生大鼠原代心肌细胞，接种于聚乳酸支架上，在转瓶中搅拌培养24小时，然后转移至旋转细胞培养系统中进行三维培养。在倒置相差显微镜、扫描电子显微镜和透射电子显微镜下观察心肌细胞的生长情况，并通过MTT法评估代谢活性。
具有持续代谢活性的心肌细胞附着于聚乳酸支架上，伸展并融合。在某些区域发现了聚乳酸-心肌细胞的搏动。
旋转细胞培养系统适用于新生大鼠心肌细胞的三维建模培养。
