Auger J, Yang W, Arnault I, Pannier F, Potin-Gautier M
I.R.B.I. UMR 6035 CNRS, Université F. Rabelais, Parc de Grandmont, 37200 Tours, France.
J Chromatogr A. 2004 Apr 2;1032(1-2):103-7. doi: 10.1016/j.chroma.2003.11.077.
Garlic and onion, are well known for their medical value, especially in against cancer and anticardiovacular diseases. "Alliins" (S-alk(en)yl-L-cysteine sulphoxides) are sources of major active compounds in Allium plants. Se incorporation into garlic significantly increases activities of garlic in cancer prevention and inhibition. Selenomethionine, selenocysteine and Se-methylselenocysteine have been identified in garlic and onion. Previously we identified gamma-glutamyl-Se-methyl-L-selenocysteine, in extracts of garlic cultivated in Se-rich soil [Med. Res. Rev. 16 (1) (1996) 111], suggesting the possible existence of Se-alk(en)yl-L-cysteine selenoxides (Se-"alliins") in garlic. Several comparative experiments were carried out to demonstrate the existence of Se-"alliins" in Se-enriched garlic and onion. We found that there was one similar time-dependent Se signal in HPLC-inductively coupled plasma MS chromatograms of cold-water extracts of freeze-dried garlic powder and fresh garlic. This signal was lost when the extracts of garlic powder and fresh garlic were stored for 1 day at >4 degrees C, but remained in fresh onion extract at the same storage conditions. These phenomena and possible mechanisms are discussed. An additional experiment showed that Allium species cultivated in Se-rich soil might contain two different Se-"alliins".
大蒜和洋葱因其医学价值而闻名，尤其是在抗癌和抗心血管疾病方面。“蒜氨酸”（S-烷（烯）基-L-半胱氨酸亚砜）是葱属植物中主要活性化合物的来源。将硒掺入大蒜中可显著提高其在癌症预防和抑制方面的活性。在大蒜和洋葱中已鉴定出硒代蛋氨酸、硒代半胱氨酸和硒甲基硒代半胱氨酸。此前我们在富硒土壤中种植的大蒜提取物中鉴定出了γ-谷氨酰基-硒甲基-L-硒代半胱氨酸[《医学研究评论》16 (1) (1996) 111]，这表明大蒜中可能存在硒-烷（烯）基-L-半胱氨酸硒氧化物（硒-“蒜氨酸”）。进行了多项对比实验以证明富硒大蒜和洋葱中存在硒-“蒜氨酸”。我们发现，冻干蒜粉和新鲜大蒜的冷水提取物的高效液相色谱-电感耦合等离子体质谱色谱图中存在一个类似的随时间变化的硒信号。当蒜粉和新鲜大蒜的提取物在4℃以上储存1天时，该信号消失，但在相同储存条件下新鲜洋葱提取物中该信号仍然存在。对这些现象及其可能的机制进行了讨论。另一项实验表明，在富硒土壤中种植的葱属植物可能含有两种不同类型的硒-“蒜氨酸”。
