Vesey D A, Selden A C, Hodgson H J
Department of Medicine, Royal Postgraduate Medical School, London, United Kingdom.
J Hepatol. 1992 May;15(1-2):107-13. doi: 10.1016/0168-8278(92)90020-p.
We investigated the changes in cell surface epidermal growth factor (EGF) receptors in the liver after partial hepatectomy, and in primary adult rat hepatocyte cultures following stimulation with either EGF, or a preparation of hepatocyte growth factor, or an insulin-glucagon combination. We confirmed a reduction in EGF receptors on hepatocytes after partial hepatectomy and a rapid down-regulation of EGF receptors on normal hepatocytes in vitro following exposure to EGF. Insulin and glucagon and hepatocyte growth factor, whilst initiating hepatocyte DNA synthesis, had only slight effects on their EGF binding capacity and EGF-receptor affinity. These results indicate that changes in cell membranes early in proliferation have only non-specific effects on EGF receptors, and, therefore, support the role of ligand binding to the EGF receptor as an important component of hepatocyte proliferation in vivo.
我们研究了部分肝切除术后肝脏中细胞表面表皮生长因子（EGF）受体的变化，以及原代成年大鼠肝细胞培养物在受到EGF、肝细胞生长因子制剂或胰岛素-胰高血糖素组合刺激后的变化。我们证实，部分肝切除术后肝细胞上的EGF受体减少，体外正常肝细胞在暴露于EGF后，其EGF受体迅速下调。胰岛素、胰高血糖素和肝细胞生长因子虽然能启动肝细胞DNA合成，但对其EGF结合能力和EGF受体亲和力只有轻微影响。这些结果表明，增殖早期细胞膜的变化对EGF受体只有非特异性影响，因此，支持配体与EGF受体结合在体内肝细胞增殖中起重要作用的观点。
