Wellenberg G J, Stockhofe-Zurwieden N, de Jong M F, Boersma W J A, Elbers A R W
Department of Mammalian Virology, Division of Infectious Diseases and Food Chain Quality, Institute for Animal Science and Health (ID-Lelystad), P.O. Box 65, 8200 AB Lelystad, The Netherlands.
Vet Microbiol. 2004 Apr 19;99(3-4):203-14. doi: 10.1016/j.vetmic.2004.01.001.
In a case-control study, the role of porcine circovirus 2 (PCV2) and putative co-factors in the development of porcine dermatitis and nephropathy syndrome (PDNS) were investigated. Pigs with and without PDNS were examined for macroscopic lesions and histopathology. In addition, organs and tissues were collected at necropsy and examined for the presence of fibrinous deposits (immune complexes), CD8+ cells, and for the presence of bacterial and viral infections. Results from PDNS cases were compared with those of three control groups comprising pigs without clinical signs of PDNS and selected from; (1) the same compartment as PDNS cases, (2) another compartment but in the same PDNS herd, and (3) a control herd without any history of PDNS or post-weaning multisystemic wasting syndrome. Macroscopic and histopathological lesions found in PDNS cases were comparable to those previously documented for PDNS e.g. skin lesions and renal lesions representing glomerulonephritis associated with fibrinous deposits and to a lesser extent with interstitial nephritis. PCV2 was detected by PCR in 100% of the PDNS cases, mainly in lymph nodes and tonsils, and in 63% of the control pigs from PDNS free herds. Virus isolation did not reveal infectious PCV2 in all cases. In PDNS affected pigs the PCV2 serum antibody titres were consistently extremely high and the mean PCV2 antibody titre in PDNS pigs was significantly higher than the mean PCV2 antibody titres in pigs from all 3 control groups. Immunohistochemical investigation of kidneys from PDNS affected pigs revealed an increased accumulation of IgG1 + IgG2 and IgM, the complement factors C1q and C3, but also an increase of CD8+ cells. The amounts of IgA and the complement factor C5 in kidneys of PDNS pigs were only slightly increased as compared to control pigs. This study demonstrates that PCV2 infections can result in extremely high PCV2 antibody titres and that PCV2 is a candidate as primary agent in the development of PDNS. The causative physiological basis for PDNS may be the excessive levels of PCV2 antibodies.
在一项病例对照研究中，对猪圆环病毒2型（PCV2）及假定的辅助因子在猪皮炎肾病综合征（PDNS）发生发展中的作用进行了研究。对患有和未患PDNS的猪进行了肉眼病变和组织病理学检查。此外，在尸检时收集器官和组织，检查是否存在纤维蛋白沉积物（免疫复合物）、CD8 +细胞以及细菌和病毒感染情况。将PDNS病例的结果与三个对照组的结果进行比较，这三个对照组的猪均无PDNS的临床症状，且分别选自：（1）与PDNS病例在同一栏舍；（2）另一栏舍但在同一PDNS猪群中；（3）无任何PDNS或断奶后多系统消耗综合征病史的对照猪群。PDNS病例中发现的肉眼和组织病理学病变与先前记录的PDNS病变相似，例如皮肤病变和肾脏病变，表现为与纤维蛋白沉积物相关的肾小球肾炎，在较小程度上伴有间质性肾炎。通过PCR在100%的PDNS病例中检测到PCV2，主要存在于淋巴结和扁桃体中，在来自无PDNS猪群的对照猪中，63%检测到PCV2。病毒分离在所有病例中均未发现具有感染性的PCV2。在受PDNS影响的猪中，PCV2血清抗体滴度一直极高，且PDNS猪的平均PCV2抗体滴度显著高于所有三个对照组猪的平均PCV2抗体滴度。对受PDNS影响的猪的肾脏进行免疫组织化学研究发现，IgG1 + IgG2和IgM、补体因子C1q和C3的积累增加，但CD8 +细胞也增加。与对照猪相比，PDNS猪肾脏中IgA和补体因子C5的量仅略有增加。本研究表明，PCV2感染可导致极高的PCV2抗体滴度，且PCV2是PDNS发生发展中的主要病原体候选。PDNS的致病生理基础可能是PCV2抗体水平过高。
