意大利撒丁岛绵羊和山羊中伯氏考克斯氏体的发生、分布及其在流产中的作用

Occurrence, distribution, and role in abortion of Coxiella burnetii in sheep and goats in Sardinia, Italy.

作者信息

Masala Giovanna, Porcu Rosaura, Sanna Giovanna, Chessa Giovanna, Cillara Grazia, Chisu Valentina, Tola Sebastiana

机构信息

Istituto Zooprofilattico Sperimentale della Sardegna G. Pegreffi, Via Duca degli Abruzzi 8, 07100 Sassari, Italy.

出版信息

Vet Microbiol. 2004 Apr 19;99(3-4):301-5. doi: 10.1016/j.vetmic.2004.01.006.

DOI:10.1016/j.vetmic.2004.01.006

PMID:15066733

Abstract

Between 1999 and 2002, 9349 sera and 517 aborted samples (422 foetuses and 95 placenta) were analysed from 675 sheep and 82 goat farms distributed all over the island of Sardinia. After abortion notification, sera collected at random from adult animals were examined to detect antibodies specific to Coxiella burnetii by ELISA, whereas foetuses and placenta were analysed by PCR assay. Specific IgG antibodies were detected in 255 (38%) sheep farms and in 39 (47%) goat herds whereas 40 ovine (10%) and 3 (6%) caprine foetuses were C. burnetii PCR-positive. Although C. burnetii DNA was amplified from different types of tissues, placenta was the tissue with the highest detection rate. Seroprevalence analysis indicates that C. burnetii distribution in sheep and goats is very high, but PCR results demonstrate that C. burnetii has a relatively low role in abortion, especially in goats.

摘要

1999年至2002年期间，对撒丁岛各地675个绵羊养殖场和82个山羊养殖场的9349份血清以及517份流产样本（422个胎儿和95份胎盘）进行了分析。接到流产通报后，通过ELISA检测从成年动物中随机采集的血清，以检测针对伯氏考克斯体的特异性抗体，而胎儿和胎盘则通过PCR检测进行分析。在255个（38%）绵羊养殖场和39个（47%）山羊养殖场中检测到特异性IgG抗体，而40个（10%）绵羊胎儿和3个（6%）山羊胎儿的伯氏考克斯体PCR检测呈阳性。尽管从不同类型的组织中扩增出了伯氏考克斯体DNA，但胎盘是检测率最高的组织。血清阳性率分析表明，伯氏考克斯体在绵羊和山羊中的分布非常高，但PCR结果表明，伯氏考克斯体在流产中所起的作用相对较小，尤其是在山羊中。