使用BacT/Alert系统在全胃肠外营养（TPN）中检测到近平滑念珠菌，并通过随机扩增多态性DNA进行鉴定。
Candida parapsilosis detected in TPN using the BacT/Alert system and characterized by randomly amplified polymorphic DNA.
作者信息
Marais E, Stewart R, Dusé A G, Rosekilly I C, de Jong G, Aithma N
机构信息
Department of Clinical Microbiology and Infectious Diseases, National Health Laboratory Services and University of the Witwatersrand, Johannesburg, PO Box 2115, Houghton 2041, South Africa.
出版信息
J Hosp Infect. 2004 Apr;56(4):291-6. doi: 10.1016/j.jhin.2004.01.014.
Candida parapsilosis was detected in environmental swabs and batches of total parenteral nutrition (TPN) products after routine monitoring. The isolates were analysed using randomly amplified polymorphic DNA (RAPD) to determine clonality and establish the most likely source of contamination. Of 20 isolates analysed, 18 were indistinguishable clonally and were found to be associated with particular work stations. The application of regular testing using a system such as the BacT/Alert, and molecular studies for epidemiological analysis, is of benefit to producers of medical products such as TPN to ensure patient safety.
在常规监测后，在环境拭子和多批全胃肠外营养（TPN）产品中检测到近平滑念珠菌。使用随机扩增多态性DNA（RAPD）对分离株进行分析，以确定克隆性并确定最可能的污染源。在分析的20株分离株中，18株在克隆上无法区分，并发现与特定工作站有关。使用诸如BacT/Alert系统进行定期检测以及进行分子研究以进行流行病学分析，对TPN等医疗产品生产商确保患者安全是有益的。
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