Li Yan-hua, Sun Shan-quan
Department of Neurobiology, Chongqing University of Medical Science, Chongqing 400016, China.
Zhongguo Wei Zhong Bing Ji Jiu Yi Xue. 2004 Apr;16(4):210-3.
To investigate the expression of aquaporin 4(AQP4) mRNA and the role in cultured rat astrocytes after exposured to hypertonic medium.
Rat cerebral cortical astrocytes from 2-day newborn Wistar rats were seperated for the pure culture in vitro and the model of cell dehydration was established by exposed cultured astrocytes to hypertonic medium. The reactivity and rules of AQP4 mRNA expression of astrocytes to hypertonic medium were studied by in situ hybridization, the activity of lactate dehydrogenase (LDH) was detected, and image was analyzed.
After astrocytes cultured for 3, 6, 12, 24 hours with hypertonic medium (320, 333 and 345 mmol/L), the activity of LDH showed a significant increase than that in the control group after astrocytes exposed to hypertonic medium (all P<0.05). In situ hybridization analysis confirmed that the expression of AQP4 mRNA was markedly increased, and especially it was significantly increased in hypertonic medium for 12 hours.
The cell viability is significantly decreased and the expression of AQP4 mRNA is remarkably increased in hypertonic medium. The result suggests that AQP4 plays an important role in the early stage of the hypertonic dehydration.
探讨高渗培养基作用后大鼠培养星形胶质细胞中水通道蛋白4（AQP4）mRNA的表达及其作用。
取2日龄新生Wistar大鼠大脑皮质星形胶质细胞进行体外纯培养，将培养的星形胶质细胞置于高渗培养基中建立细胞脱水模型。采用原位杂交技术研究星形胶质细胞对高渗培养基的反应性及AQP4 mRNA表达规律，检测乳酸脱氢酶（LDH）活性并进行图像分析。
星形胶质细胞在高渗培养基（320、333和345 mmol/L）中培养3、6、12、24小时后，与对照组相比，高渗培养基作用后星形胶质细胞LDH活性显著升高（均P<0.05）。原位杂交分析证实，AQP4 mRNA表达明显增加，尤其是在高渗培养基中作用12小时时显著增加。
高渗培养基作用下细胞活力显著降低，AQP4 mRNA表达明显增加。结果提示AQP4在高渗性脱水早期发挥重要作用。
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