Fu Xue-Mei, Yao Yu-Jia, Yang Zhao, Xiang Long, Gao Ju
Department of Pediatrics, West China Second Hospital, Sichuan University, Chengdu 610041, China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2005 Sep;36(5):641-4.
To investigate the expression of aquaorin-4 (AQP4) in the cultured neonatal rat primary astrocytes during hypoxia and during reoxygenation after hypoxia.
The astrocytes were randomly assigned to the hypoxia group (the astrocytes were exposed to hypoxia for 3 h, 6 h, 12 h and 24 h respectively), the reoxygenation group (after exposure to 12 h hypoxia, the astrocytes were returned to normoxic cultures for 3 h, 6 h, 12 h and 24 h respectively), and the normoxic group (control group). AQP4 mRNA expression in astrocytes of all groups was detected by Real Time PCR, and AQP4 protein expression was determined by immunofluorescence.
The AQP4 mRNA and protein expressions significantly decreased with the time of hypoxia, and during reoxygenation, the decreased expressions were gradually up-reguated.
It is likely that the decreased expression of AQP4 is associated with brain edema and in such brain edema conditions AQP4 may play a prominent role in the reestablishment of brain osmotic equilibrium.
研究水通道蛋白4（AQP4）在新生大鼠原代培养星形胶质细胞缺氧及缺氧复氧过程中的表达情况。
将星形胶质细胞随机分为缺氧组（分别缺氧3小时、6小时、12小时和24小时）、复氧组（缺氧12小时后，再分别复氧培养3小时、6小时、12小时和24小时）和常氧组（对照组）。采用实时荧光定量PCR检测各组星形胶质细胞中AQP4 mRNA的表达，免疫荧光法检测AQP4蛋白的表达。
随着缺氧时间的延长，AQP4 mRNA和蛋白表达显著降低，在复氧过程中，降低的表达逐渐上调。
AQP4表达降低可能与脑水肿有关，在这种脑水肿情况下，AQP4可能在脑渗透压平衡重建中起重要作用。
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