Galloway Darrell, Liner Adriane, Legutki Joseph, Mateczun Alfred, Barnewall Roy, Estep James
Department of Microbiology, The Ohio State University, 484 West 12th Avenue, Columbus, OH 43210, USA.
Vaccine. 2004 Apr 16;22(13-14):1604-8. doi: 10.1016/j.vaccine.2003.09.043.
The objective of this study was to determine whether a DNA prime-protein boost immunization against the Bacillus anthracis protective antigen (PA) and lethal factor (LF) antigens could induce a protective immune response against significant aerosol challenge in the rabbit model. Rabbits were vaccinated with different regimens of DNA vaccines (Table 1) and aerosol challenged with B. anthracis spores, Ames strain, with an average dose of 50 LD(50s) with a range from 18 to 169 LD(50s.) Of the five vaccinated rabbits that survived, two were immunized intramuscularly (i.m.) with DNA followed with a protein boost and three were immunized subcutaneous (s.q.) with recombinant protein. A major factor predicting survival was the ability of the animal to mount a lasting antibody response to PA. Rabbit sera were collected prior to and following aerosol challenge and titrated for PA antibodies by indirect ELISA. The results of this study indicate that DNA-based immunization against PA and LF induces significant protective immunity against aerosol challenge in the rabbit model and compares favorably with protein-based immunization.
本研究的目的是确定针对炭疽芽孢杆菌保护性抗原（PA）和致死因子（LF）抗原的DNA初免-蛋白质加强免疫能否在兔模型中诱导针对显著气溶胶攻击的保护性免疫反应。用不同方案的DNA疫苗对兔子进行免疫接种（表1），并用炭疽芽孢杆菌孢子（Ames菌株）进行气溶胶攻击，平均剂量为50个半数致死量（LD50），范围为18至169个LD50。在存活的5只接种疫苗的兔子中，2只通过肌肉注射（i.m.）DNA随后进行蛋白质加强免疫，3只通过皮下（s.q.）接种重组蛋白进行免疫。预测存活与否的一个主要因素是动物对PA产生持久抗体反应的能力。在气溶胶攻击之前和之后收集兔血清，并通过间接ELISA法测定PA抗体。本研究结果表明，针对PA和LF的基于DNA的免疫接种在兔模型中可诱导针对气溶胶攻击的显著保护性免疫，与基于蛋白质的免疫接种相比具有优势。
