Arana-Cuenca Ainhoa, Roda Arantxa, Téllez Alejandro, Loera Octavio, Carbajo José M, Terrón María C, González Aldo E
Centro de Investigaciones Biológicas CSIC, Ramiro de Maeztu 9, 28040 Madrid Spain.
J Basic Microbiol. 2004;44(2):79-87. doi: 10.1002/jobm.200310324.
The study of isozymes has to date been successfully applied in the identification of a number of different microorganisms, particularly fungi. Trametes sp. I-62 (CECT20197) is a white-rot fungus with potential biotechnological applications. It has been identified as Trametes on the basis of the mycelium morphology, but the features observed are not sufficient for its classification at the species level. Given that this strain produces a very characteristic laccase isozyme pattern, in the present work we compared its isozyme profile with the profiles produced by ten other very closely phylogenetically related fungi from the Polyporaceae family. The data indicates that Trametes sp. I-62 could not be included in any of the taxonomically related species which were analysed in the present work. Extracellular laccases were detected in nine of the fungi studied and in four of them namely Trametes subectypus, Trametes pavonia, Trametes ochracea and Trametes gallica, this is the first report of such activity. Given that phylogenetically related fungi growing in the same culture conditions exhibit different laccase patterns, these isozymes could be used as an additional criterion for fungal identification.
迄今为止，同工酶研究已成功应用于多种不同微生物的鉴定，尤其是真菌。栓菌属Trametes sp. I-62（CECT20197）是一种具有潜在生物技术应用价值的白腐真菌。基于菌丝体形态，它已被鉴定为栓菌属，但所观察到的特征不足以将其在种水平上进行分类。鉴于该菌株产生非常独特的漆酶同工酶模式，在本研究中，我们将其同工酶谱与其他十种来自多孔菌科、系统发育关系非常密切的真菌所产生的谱进行了比较。数据表明，Trametes sp. I-62不能归入本研究中分析的任何一个分类学相关物种。在所研究的九种真菌中检测到了胞外漆酶，其中四种真菌，即亚拟栓菌Trametes subectypus、淡黄栓菌Trametes pavonia、赭黄栓菌Trametes ochracea和粗毛栓菌Trametes gallica，此类活性的报道尚属首次。鉴于在相同培养条件下生长的系统发育相关真菌表现出不同的漆酶模式，这些同工酶可作为真菌鉴定的额外标准。
