Mossini Simone Aparecida Galerani, de Oliveira Kesia Pires, Kemmelmeier Carlos
Department of Biochemistry, Universidade Estadual de Maringá, Maringá PR Brazil.
J Basic Microbiol. 2004;44(2):106-13. doi: 10.1002/jobm.200310332.
Aqueous extract of the leaves of neem [Azadirachta indica A. JUSS (Meliaceae)] was tested in vitro for antifungal activity against Penicillium expansum. Patulin production was inhibited during cultivation, when concentrations higher than 50 mg/ml of neem leaf extract was added to the culture medium. Analyses of mycotoxin production were performed by TLC and HPLC. Fungal growth and colony characteristics, in the presence of the extract, were investigated and compared with extract-free cultures.
对印楝（楝科植物印楝树Azadirachta indica A. JUSS）叶片的水提取物进行了体外抗扩展青霉的抗真菌活性测试。当向培养基中添加浓度高于50 mg/ml的印楝叶提取物时，培养过程中展青霉素的产生受到抑制。通过薄层色谱法（TLC）和高效液相色谱法（HPLC）对霉菌毒素的产生进行分析。研究了提取物存在时真菌的生长和菌落特征，并与无提取物的培养物进行比较。
