丝状真菌绿色木霉营养菌丝体中的氯离子转运

Chloride transport in the vegetative mycelia of filamentous fungus Trichoderma viride.

作者信息

Simkovic M, Pokorný R, Hudecová D, Varecka L

机构信息

Department of Biochemistry and Microbiology, Faculty of Food and Chemical Technology, Slovak University of Technology, Radlinského 9, 812 37-Bratislava, Slovakia.

出版信息

J Basic Microbiol. 2004;44(2):122-8. doi: 10.1002/jobm.200310261.

DOI:10.1002/jobm.200310261

PMID:15069671

Abstract

The influx of chlorides into Trichoderma viride vegetative submerged mycelium was measured by means of the radionuclide (36)Cl(-). It was found that the (36)Cl(-) influx was time-dependent (the steady-state was established with t(1/2 )= 25 min at 25 degrees C), pH-dependent (with pH optimum between 4-5.5), temperature-dependent (at about 15 degrees C), and concentration-dependent (K(M)(Cl(-))) = 47.6 +/- 4.2 micromol x l(-1); J(max) = 11.5 +/- 0.7 pmol(Cl(-)) x min(-1). mg(dry mass) (-1)). The (36)Cl(-) influx was inhibited by Br(-) but not F(-), I(-), SO(4)(2-), HPO(3)(2-) and HCO(3)(-). The presence of vanadate (P-type ATPase inhibitor) moderately stimulated the (36)Cl(-) influx but the presence valinomycin (electrogenic K(+) ionophore), salicylate (known to release Ca(2+) from Trichoderma viride internal stores) were without effect on the (36)Cl(-) influx. The results suggest that the (36)Cl(-) influx is mediated by a carrier and that the transport is electroneutral, probably Cl(-)/OH(-) antiport.

摘要

采用放射性核素（³⁶）Cl⁻测定了氯化物流入绿色木霉营养型深层菌丝体的情况。结果发现，³⁶Cl⁻流入具有时间依赖性（在25℃下，t₁/₂ = 25分钟时达到稳态）、pH依赖性（最适pH在4 - 5.5之间）、温度依赖性（约15℃）和浓度依赖性（Kₘ(Cl⁻) = 47.6 ± 4.2 μmol·L⁻¹；Jₘₐₓ = 11.5 ± 0.7 pmol(Cl⁻)·min⁻¹·mg（干质量）⁻¹）。³⁶Cl⁻流入受到Br⁻的抑制，但不受F⁻、I⁻、SO₄²⁻、HPO₃²⁻和HCO₃⁻的影响。钒酸盐（P型ATP酶抑制剂）的存在适度刺激了³⁶Cl⁻流入，但缬氨霉素（生电性K⁺离子载体）、水杨酸盐（已知可从绿色木霉内部储存库释放Ca²⁺）的存在对³⁶Cl⁻流入没有影响。结果表明，³⁶Cl⁻流入是由载体介导的，且转运是电中性的，可能是Cl⁻/OH⁻反向转运。