de Souza Cristina Giatti Marques, Tychanowicz Giovana Kirst, de Souza Daniela Farani, Peralta Rosane Marina
Departamento de Bioquímica, Universidade Estadual de Maringá, 87020900, Maringá, PR, Brazil.
J Basic Microbiol. 2004;44(2):129-36. doi: 10.1002/jobm.200310365.
The effect of several phenolic and aromatic monomers structurally-related to lignin on production of laccase by the white rot fungus P. pulmonarius (Fr.) Quélet has been studied. In the absence of an inducer, laccase was maximally produced after depletion of carbon and nitrogen sources. Among 15 phenolic and aromatic compounds tested, ferulic acid and vanillin were the most efficient inducers, increasing the production of laccase activity up to 10 times. A mixture of ferulic acid and vanillin was more efficient to induce the production of laccase than the isolated phenolics. At least three laccase isoforms designated as lcc1, lcc2 and lcc3 were identified by eletrophoretic analysis of P. pulmonarius culture filtrates. The lcc1 and lcc2 isoforms were produced by non-induced cultures, while lcc3 was found only in induced-culture filtrates.
研究了几种与木质素结构相关的酚类和芳香族单体对白腐真菌肺形侧耳（P. pulmonarius (Fr.) Quélet）漆酶产生的影响。在没有诱导剂的情况下，碳源和氮源耗尽后漆酶产量最高。在所测试的15种酚类和芳香族化合物中，阿魏酸和香草醛是最有效的诱导剂，可使漆酶活性产量提高至10倍。阿魏酸和香草醛的混合物比单独的酚类更有效地诱导漆酶的产生。通过对肺形侧耳培养滤液的电泳分析，鉴定出至少三种漆酶同工酶，分别命名为lcc1、lcc2和lcc3。lcc1和lcc2同工酶由未诱导的培养物产生，而lcc3仅在诱导培养滤液中发现。
