Popov V I, Deev A A, Klimenko O A, Kraev I V, Kuz'minykh S B, Medvedev N I, Patrushev I V, Popov R V, Rogachevskiĭ V V, Khutsian S S, Stewart M G, Fesenko E E
Institute of Cell Biophysics, Institute of Theoretical and Experimental Byophysics, Branch of Shemyakin, Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Pushchino, Russia.
Zh Vyssh Nerv Deiat Im I P Pavlova. 2004 Jan-Feb;54(1):120-9.
The article reviews the literature data and results obtained by the authors concerning synaptic plasticity and remodeling of synaptic organelles in the central nervous system. Modern techniques of laser scanning confocal microscopy and serial thin sectioning for in vivo and in vitro studies of dendritic spines including a correlation between morphological changes and synaptic transmission efficiency are discussed, particularly, in relation to long-term potentiation. Organization of different types of dendritic spines and involvement of filopodia in spine genesis are examined. Significance of serial ultrathin sections for unbiased quantitative stereological analysis and three-dimensional reconstructions is discussed. The contact of one dendritic spine with two presynaptic boutons (multiple synapses) on both CA1 mushroom dendritic spines is discussed. The analyzed findings suggest new ideas for organization and functioning of synapses.
本文回顾了作者们关于中枢神经系统中突触可塑性和突触细胞器重塑的文献数据及研究结果。讨论了用于体内和体外研究树突棘的激光扫描共聚焦显微镜和连续超薄切片等现代技术，尤其涉及形态学变化与突触传递效率之间的相关性，特别是与长时程增强的关系。研究了不同类型树突棘的组织形式以及丝状伪足在树突棘形成中的作用。讨论了连续超薄切片对于无偏倚定量立体分析和三维重建的意义。探讨了CA1蘑菇状树突棘上一个树突棘与两个突触前终扣（多个突触）的接触情况。分析结果为突触的组织和功能提出了新观点。
