Spencer P, Trylovich D J, Cobb C M
Department of Pediatric Dentistry, University of Missouri-Kansas City School of Dentistry.
J Periodontol. 1992 Jul;63(7):633-6. doi: 10.1902/jop.1992.63.7.633.
Recently lasers have been recommended as an alternative or adjunctive therapy in the control and treatment of periodontally diseased root surfaces. The purpose of this in vitro investigation was to characterize the chemical structure of lased root surfaces using Fourier transform infrared photoacoustic spectroscopy (FTIR/PAS). Cementum samples, 6 mm x 2 mm, were cut from the root surface of extracted non-carious, unerupted human molars. The experimental samples were lased with a Nd:YAG laser at an average energy of 80 mJ at 10 pulses per second. Total lasing time ranged from 1 minute 45 seconds to 4 minutes. A non-lased cementum sample served as the control. All spectra were recorded from 4000 to 400 cm-1 using the photoacoustic cell attachment on an Analect RFX-65 FTIR Spectrometer. Photoacoustic FTIR spectra of lased cementum samples showed a decrease in the protein/mineral ratio in comparison to the control. Breakdown of protein at the root surface potentially contributed to an ammonium band at 2010 cm-1. The decreased protein/mineral ratio and the potential surface contamination with protein by-products, may ultimately affect cell reattachment at the cementum surface.
最近，激光已被推荐作为控制和治疗牙周病牙根表面的一种替代或辅助疗法。本体外研究的目的是使用傅里叶变换红外光声光谱法（FTIR/PAS）对激光照射后的牙根表面化学结构进行表征。从拔除的无龋、未萌出的人类磨牙牙根表面切取6mm×2mm的牙骨质样本。实验样本用钕钇铝石榴石（Nd:YAG）激光照射，平均能量为80mJ，每秒10个脉冲。总照射时间为1分45秒至4分钟。一个未照射的牙骨质样本作为对照。使用Analect RFX-65傅里叶变换红外光谱仪上的光声池附件，在4000至400cm-1范围内记录所有光谱。与对照相比，激光照射后的牙骨质样本的光声傅里叶变换红外光谱显示蛋白质/矿物质比率降低。牙根表面蛋白质的分解可能导致在2010cm-1处出现铵带。蛋白质/矿物质比率的降低以及蛋白质副产物可能造成的表面污染，最终可能会影响细胞在牙骨质表面的重新附着。
