Hara-Kuge Sayuri, Seko Akira, Shimada Osamu, Tosaka-Shimada Hisami, Yamashita Katsuko
Department of Biochemistry, Sasaki Institute, 2-2 Kanda-Surugadai, Chiyoda-ku, Tokyo 101-0062, Japan.
Glycobiology. 2004 Aug;14(8):739-44. doi: 10.1093/glycob/cwh082. Epub 2004 Apr 7.
Vesicular integral protein of 36 kDa (VIP36) is an intracellular lectin recognizing high-mannose type glycans and is highly expressed in salivary glands, especially the parotid gland, which secretes alpha-amylase in large quantities. Here immunoelectron microscopy demonstrated that VIP36 was primarily localized to secretory vesicles in the glandula parotis of the rat, where alpha-amylase also resided. A secretory vesicle fraction, prepared by Percoll density gradient centrifugation, contained both VIP36 and alpha-amylase. Moreover, alpha-amylase that was localized to these secretory vesicles contained high-mannose type glycans. In addition, VIP36 coprecipitated with alpha-amylase in an endo H treatment-sensitive manner. These results suggest that VIP36 is involved in the secretion of alpha-amylase in the rat parotid gland.
36 kDa囊泡整合蛋白（VIP36）是一种识别高甘露糖型聚糖的细胞内凝集素，在唾液腺尤其是腮腺中高表达，腮腺大量分泌α-淀粉酶。在此，免疫电子显微镜显示VIP36主要定位于大鼠腮腺的分泌小泡中，α-淀粉酶也存在于此处。通过Percoll密度梯度离心制备的分泌小泡组分同时含有VIP36和α-淀粉酶。此外，定位于这些分泌小泡中的α-淀粉酶含有高甘露糖型聚糖。另外，VIP36与α-淀粉酶以内切糖苷酶H处理敏感的方式共沉淀。这些结果表明VIP36参与大鼠腮腺中α-淀粉酶的分泌。
