Zhu Bin, Wan Chao-min, Guo Hui-ling, Xiao Jing, Wang Zheng-rong
Department of Biomedical Engineering, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2004 Mar;35(2):179-81.
To investigate the changes of myosin ATPase activities in cardiac ischemic reperfusion injury of rabbit.
A convenient method was adopted to purify the myosin from the left ventricle of normal rabbit heart and of cardiac ischemic reperfusion injured rabbit heart. The michaelis constant (K(m)) and maximum velocity(Vmax) values of Ca(2+)-activated ATPase were determined from the rate of Pi release in enzymatic reaction. The myosin ATPase activities of normal group and ischemic group were compared.
The myosin ATPase activities of the control group were Vmax: (1.15 +/- 0.17) mumol/(mg.min), K(m): (5.43 +/- 2.18) mmol and those of ischemic group were Vmax: (1.17 +/- 0.21) mumol/(mg.min), K(m): (6.02 +/- 2.01) mmol. There was no significant difference between the two groups(P > 0.05).
This modelling animal experiment showed that the myosin ATPase activities were unchanged in cardiac ischemic reperfusion injury.
探讨兔心脏缺血再灌注损伤时肌球蛋白ATP酶活性的变化。
采用简便方法从正常兔心脏左心室及心脏缺血再灌注损伤兔心脏中纯化肌球蛋白。根据酶促反应中无机磷酸（Pi）释放速率测定钙激活ATP酶的米氏常数（K(m)）和最大反应速度（Vmax）值。比较正常组和缺血组的肌球蛋白ATP酶活性。
对照组肌球蛋白ATP酶活性为Vmax：（1.15±0.17）μmol/（mg·min），K(m)：（5.43±2.18）mmol；缺血组为Vmax：（1.17±0.21）μmol/（mg·min），K(m)：（6.02±2.01）mmol。两组间差异无统计学意义（P＞0.05）。
本动物模型实验表明，心脏缺血再灌注损伤时肌球蛋白ATP酶活性未发生改变。
