[大蝰蛇和角蝰蛇毒液对癌细胞（IGR39、HT29-D4和IGROV1）整合素粘附的影响]

[Effect of Macrovipera lebetina and Cerastes cerastes venoms on adherence to integrins of cancerous cells (IGR39, HT29-D4 and IGROV1)].

作者信息

Marrakchi N, Sarray S, Marvaldi J, El Ayeb M, Luis J

机构信息

Laboratoire des Venins et Toxines, Institut Pasteur de Tunis.

出版信息

Arch Inst Pasteur Tunis. 2002;79(1-4):3-9.

PMID:15072239

Abstract

In this work, we provide experimental arguments in favor of the fact that components from Macrovipera lebetina and Cerastes cerastes venoms bind to IGR39 melanoma cells but not to HT29D4 cells that derive from carcinoma adenome. Furthermore, Macrovipera lebetina and Cerastes cerastes venoms inhibit the adherence of IGR39 and HT 29-D4 to various extracellular matrix proteins. Macrovipera lebetina and Cerastes cerastes venoms did not inhibit the non specific adherence of IGR 39 cells to polylysine. In addition, binding of components from Cerastes cerastes venom to IGR39 cells is inhibited by GRGDS peptide and by monoclonal antibidy anti-av, while these two components have no effect on the adherence of IGR39 to Macrovipera lebetina venom.

摘要

在这项工作中，我们提供了实验依据，支持以下事实：来自黎凡特蝰蛇（Macrovipera lebetina）和角蝰（Cerastes cerastes）毒液的成分可与IGR39黑色素瘤细胞结合，但不与源自腺癌的HT29D4细胞结合。此外，黎凡特蝰蛇和角蝰毒液可抑制IGR39和HT 29-D4与各种细胞外基质蛋白的黏附。黎凡特蝰蛇和角蝰毒液不会抑制IGR 39细胞与聚赖氨酸的非特异性黏附。此外，角蝰毒液成分与IGR39细胞的结合受到GRGDS肽和抗αv单克隆抗体的抑制，而这两种成分对IGR39与黎凡特蝰蛇毒液的黏附没有影响。