Thorén S A
Department of Internal Medicine, University Hospital, Lund, Sweden.
J Toxicol Environ Health. 1992 Aug;36(4):307-18. doi: 10.1080/15287399209531641.
A short-term toxicological test has been developed using a calorimetric method. The metabolic activity, observed as the heat exchange rate, was monitored from alveolar rabbit macrophages in monolayers exposed to different metal and non-metal particles. Calorimetric activity indices and viability indices were introduced, from which toxic effects could be assessed. Manganese dioxide particles were found to be cytotoxic. In contrast, titanium dioxide particles seemed to be harmless. The results were in accordance with the cell survival found by use of a fluorescein ester staining method and measured by an image analyzer. Toxic effects from quartz in the form of increased metabolic activity of exposed cells could be detected by the calorimeter in contradiction to the use of the image analyzer. This latter result supports the hypothesis that silica particles cause chronic modification of the macrophage function and that this change in the alveolar macrophage function may be the first of a series of processes leading to pulmonary fibrosis.
已开发出一种使用量热法的短期毒理学测试。以热交换率观察到的代谢活性，在暴露于不同金属和非金属颗粒的单层兔肺泡巨噬细胞中进行监测。引入了量热活性指数和活力指数，据此可评估毒性作用。发现二氧化锰颗粒具有细胞毒性。相比之下，二氧化钛颗粒似乎无害。结果与使用荧光素酯染色法并通过图像分析仪测量的细胞存活率一致。量热仪能够检测到石英以暴露细胞代谢活性增加形式产生的毒性作用，这与图像分析仪的检测结果相反。后一结果支持了以下假设：二氧化硅颗粒会导致巨噬细胞功能的慢性改变，而肺泡巨噬细胞功能的这种变化可能是导致肺纤维化的一系列过程中的第一步。
