Schleiss Mark R, Bourne Nigel, Stroup Greg, Bravo Fernando J, Jensen Nancy J, Bernstein David I
Division of Infectious Diseases, Children's Hospital Research Foundation, Cincinnati, Ohio 45229, USA.
J Infect Dis. 2004 Apr 15;189(8):1374-81. doi: 10.1086/382751. Epub 2004 Apr 1.
Glycoprotein B (gB) has emerged as a subunit-vaccine candidate for congenital cytomegalovirus (CMV) infection, a major public health problem. The present study evaluated a cloned, recombinant gB vaccine in the guinea pig cytomegalovirus (GPCMV) model of congenital infection. Guinea pigs were immunized with gB, which was coadministered with either Freund's adjuvant or alum. All gB-immunized dams had enzyme-linked immunosorbent-assay and neutralizing-antibody responses, with significantly higher titers in the gB/Freund's group. Pregnant dams were challenged with GPCMV subcutaneously during the 3rd trimester. Maternal DNAemia on day 10 after infection trended lower in gB-immunized dams than in control animals, with significant reductions in the gB/Freund's group. Vaccination resulted in a highly significant reduction in pup mortality. For the gB-vaccine groups, pup mortality was significantly lower, and reduced rates of GPCMV transmission were noted, for dams immunized with gB and Freund's adjuvant, compared with dams immunized with gB and alum. These are the first data indicating that a recombinant gB vaccine protects against congenital CMV infection and disease.
糖蛋白B（gB）已成为先天性巨细胞病毒（CMV）感染的亚单位疫苗候选物，这是一个重大的公共卫生问题。本研究在先天性感染的豚鼠巨细胞病毒（GPCMV）模型中评估了一种克隆的重组gB疫苗。用gB免疫豚鼠，gB与弗氏佐剂或明矾共同给药。所有接受gB免疫的母鼠都有酶联免疫吸附测定和中和抗体反应，gB/弗氏佐剂组的滴度显著更高。在妊娠晚期，对怀孕母鼠进行皮下GPCMV攻击。感染后第10天，接受gB免疫的母鼠的母体DNA血症趋势低于对照动物，gB/弗氏佐剂组有显著降低。疫苗接种导致幼崽死亡率显著降低。对于gB疫苗组，与用gB和明矾免疫的母鼠相比，用gB和弗氏佐剂免疫的母鼠的幼崽死亡率显著更低，并且GPCMV传播率降低。这些是表明重组gB疫苗可预防先天性CMV感染和疾病的首批数据。
