Uji Y, Sugiuchi H, Okabe H
Department of Laboratory Medicine, Kumamoto University Medical School.
Rinsho Byori. 1992 Jul;40(7):775-82.
A monoclonal solid phase enzyme immunoassay has been developed for the detection of human troponin T. The serum troponin T levels in healthy subjects gave 0.05 +/- 0.06 ng/ml in total (n = 176), 0.06 +/- 0.07 ng/ml in males (n = 79) and 0.03 +/- 0.05 ng/ml in females. Within-run and between-run precision (CVs) of the assay were less than 5%. Various common interferents tested did not affect on the assay, but higher titer of rheumatoid factor, and anti-coagulants such as EDTA, heparin oxalate and citrate affected the assay. In all patients with defined acute myocardial infarction, serum troponin T levels increased 7 to 10 folds the upper reference range within 6 hours after the onset of chest pains and maximum elevation of serum troponin T level was at around 20 hours and its levels remained elevated for 7 to 20 days. Specificity and sensitivity for acute myocardial infarction was 92.4% and 100%, respectively. The results indicated that troponin T measurement improved the diagnostic efficiency for the detection of myocardial necrosis as compared with conventionally used cardiac enzymes and was an effective tool for the confirmation of the reperfusion by PTCA and PTCR.
已开发出一种单克隆固相酶免疫测定法用于检测人肌钙蛋白T。健康受试者血清肌钙蛋白T水平总体为0.05±0.06 ng/ml（n = 176），男性为0.06±0.07 ng/ml（n = 79），女性为0.03±0.05 ng/ml。该测定法的批内和批间精密度（CV）均小于5%。所测试的各种常见干扰物对测定无影响，但类风湿因子高滴度以及抗凝剂如乙二胺四乙酸（EDTA）、草酸肝素和柠檬酸盐会影响测定。在所有明确诊断为急性心肌梗死的患者中，胸痛发作后6小时内血清肌钙蛋白T水平升高至高于参考范围上限7至10倍，血清肌钙蛋白T水平的最大升高出现在约20小时左右，且其水平持续升高7至20天。急性心肌梗死的特异性和敏感性分别为92.4%和100%。结果表明，与传统使用的心脏酶相比，肌钙蛋白T检测提高了心肌坏死检测的诊断效率，并且是确认经皮腔内冠状动脉成形术（PTCA）和经皮冠状动脉腔内旋切术（PTCR）后再灌注的有效工具。
