Jain Sanjay Kumar, Langen Gregor, Hess Wolfgang, Börner Thomas, Hückelhoven Ralph, Kogel Karl-Heinz
Interdisciplinary Research Centre for Environmental Sciences, Institute of Phytopathology and Applied Zoology, Justus-Liebig-University Giessen, Heinrich-Buff-Ring 26, D-35392 Giessen, Germany.
Mol Plant Microbe Interact. 2004 Apr;17(4):374-82. doi: 10.1094/MPMI.2004.17.4.374.
We performed cytological and molecular analyses of the interaction between the biotrophic barley powdery mildew fungus Blumeria graminis f. sp. hordei and white and green leaves of the barley albostrians mutant. The leaves have the same nuclear genotype but differ from each other in respect to plastid differentiation. White leaves showed enhanced penetration resistance to B. graminis f. sp. hordei, associated with higher epidermal H2O2 accumulation beneath the appressorial germ tubes and protein cross-linking in papillae. Very low basal salicylic acid content was found in white leaves, which further confirmed that H2O2 accumulation and penetration resistance in barley are independent of salicylic acid. Expression analysis of stress and defense-related genes, including such being involved in reactive oxygen species production and cell death regulation, revealed stronger constitutive or pathogen-induced transcript accumulation in white leaves. We discuss the data on the basis of the finding that white albostrians leaves exhibit a supersusceptible interaction phenotype with the hemibiotrophic fungus Bipolaris sorokiniana.
我们对活体营养型大麦白粉病菌禾本科布氏白粉菌（Blumeria graminis f. sp. hordei）与大麦白化突变体的白色和绿色叶片之间的相互作用进行了细胞学和分子分析。这些叶片具有相同的核基因型，但在质体分化方面彼此不同。白色叶片对禾本科布氏白粉菌表现出增强的穿透抗性，这与附着胞萌发管下方较高的表皮过氧化氢积累以及乳突中的蛋白质交联有关。在白色叶片中发现极低的基础水杨酸含量，这进一步证实了大麦中过氧化氢的积累和穿透抗性与水杨酸无关。对包括参与活性氧产生和细胞死亡调控的基因在内的应激和防御相关基因的表达分析表明，白色叶片中组成型或病原体诱导的转录积累更强。我们基于白化叶片与半活体营养型真菌索氏平脐蠕孢（Bipolaris sorokiniana）表现出超敏感相互作用表型这一发现来讨论这些数据。
