Ferguson N M, Leiting V A, Klevena S H
Department of Avian Medicine, College of Veterinary Medicine, University of Georgia, Athens, GA 30602-4875, USA.
Avian Dis. 2004 Jan-Mar;48(1):91-9. doi: 10.1637/7069.
A Mycoplasma gallisepticum (MG) isolate from an atypically mild outbreak in turkey breeders was found to be similar to house finch isolates by DNA analyses. A preliminary study in turkeys showed that this isolate (K5054) caused very mild lesions and protected turkeys against subsequent challenge with a virulent MG strain. In this study, K5054 was further evaluated as a potential vaccine strain in commercial layer-type chickens and turkeys. The safety of K5054 was evaluated by aerosol challenge followed by evaluation of gross and histopathologic lesions as well as serologic reactions and isolation of MG from the trachea and air sacs. Infection of chickens (trial 1) and turkeys (trial 2) with K5054 resulted in little evidence of MG lesions. There was weak seroconversion, and K5054 was consistently reisolated from the tracheas of chickens and turkeys. The efficacy of K5054 as a vaccine was evaluated by aerosol challenge of vaccinated chickens (trial 3) and turkeys (trial 4) with virulent R strain. There was evidence of protection from lesions associated with MG.
通过DNA分析发现，从种火鸡非典型轻度疫情中分离出的一株鸡毒支原体（MG）与家朱雀分离株相似。在火鸡上进行的一项初步研究表明，该分离株（K5054）引起的病变非常轻微，并能保护火鸡免受强毒MG菌株的后续攻击。在本研究中，进一步评估了K5054作为商品蛋鸡和火鸡潜在疫苗株的可能性。通过气溶胶攻击评估K5054的安全性，随后评估大体和组织病理学病变以及血清学反应，并从气管和气囊中分离MG。用K5054感染鸡（试验1）和火鸡（试验2）后，几乎没有MG病变的迹象。血清转化较弱，并且能持续从鸡和火鸡的气管中重新分离出K5054。通过用强毒R株气溶胶攻击接种疫苗的鸡（试验3）和火鸡（试验4），评估K5054作为疫苗的效力。有证据表明其能预防与MG相关的病变。
