Takebayashi Shinji, Nakagawa Takayuki, Kojima Ken, Kim Tae-Soo, Kita Tomoko, Dong Youyi, Endo Tsuyoshi, Iguchi Fukuichiro, Naito Yasushi, Omori Koichi, Ito Juichi
Department of Otolaryngology--Head and Neck Surgery, Graduate School of Medicine, Kyoto University, Kyoto, Japan.
Acta Otolaryngol Suppl. 2004 Mar(551):18-21. doi: 10.1080/03655230310016753.
This study investigated the role of beta-catenin in the development of mouse auditory epithelia. Inner ears obtained from embryonic and newborn mice were used. Expression of beta-catenin was examined together with the expression of Ki-67, a marker for proliferating cells, or myosin VIIa, a marker for differentiated hair cells. In the early phase of development, intense expression of beta-catenin was found in auditory epithelia in which a number of Ki-67-positive cells were identified. Together with a decrease in proliferating cells, the intensity and area of beta-catenin expression were reduced. In addition, during differentiation and maturation of hair cells, the area of beta-catenin expression was further limited. These findings suggest that patterns of expression of beta-catenin are closely linked with the status of auditory epithelia development.
本研究调查了β-连环蛋白在小鼠听觉上皮发育中的作用。使用了从胚胎期和新生小鼠获取的内耳。检测了β-连环蛋白的表达，并同时检测了增殖细胞标志物Ki-67或分化毛细胞标志物肌球蛋白VIIa的表达。在发育早期，在听觉上皮中发现β-连环蛋白强烈表达，其中可识别出许多Ki-67阳性细胞。随着增殖细胞数量减少，β-连环蛋白表达的强度和面积均降低。此外，在毛细胞分化和成熟过程中，β-连环蛋白表达的面积进一步受限。这些发现表明，β-连环蛋白的表达模式与听觉上皮的发育状态密切相关。
