Visser Antonie J W G, Kunst Beno H, Keller Hans, Schots Arjen
MicroSpectroscopy Centre, Laboratory of Biochemistry, Wageningen University, Dreijenlaan 3, 6703 HA Wageningen, The Netherlands.
Curr Pharm Biotechnol. 2004 Apr;5(2):173-9. doi: 10.2174/1389201043376977.
The selection of specific binding molecules like peptides and proteins from biolibraries using, for instance, phage display methods can be quite time-consuming. It is therefore desirable to develop a strategy that is much faster in selection and sorting of potential binders out of a biolibrary. In this contribution we separately discuss the current achievements in generation of biolibraries, single-molecule detection techniques and microfluidic devices. A high-throughput microfluidic platform is then proposed that combines the propulsion of liquid containing fluorescent components of the biolibrary through microchannels, single-molecule fluorescence photon burst detection and real-time sorting of positive hits.
使用例如噬菌体展示方法从生物文库中筛选特定的结合分子（如肽和蛋白质）可能相当耗时。因此，需要开发一种在从生物文库中筛选和分选潜在结合物方面速度更快的策略。在本论文中，我们分别讨论了生物文库生成、单分子检测技术和微流控装置方面的当前成就。然后提出了一种高通量微流控平台，该平台将含有生物文库荧光成分的液体通过微通道推进、单分子荧光光子爆发检测以及对阳性命中物进行实时分选结合在一起。
