来自榕属植物种子的α-淀粉酶抑制剂及其对鞘翅目害虫的活性。

Alpha-amylase inhibitors from Ficus sp. seeds and their activities towards coleoptera insect pests.

作者信息

Bezerra Ingrid W L, Teixeira Fabiano M, Oliveira Adeliana S, Araújo Carina L, Leite Edda L, Queiroz Karla C S, Sales Maurício P

机构信息

Departamento de Bioquimica, Centro de Biociências, Universidade Federal do Rio Grande do Norte, 59072-970, Natal/RN, Brazil.

出版信息

Protein Pept Lett. 2004 Apr;11(2):181-7. doi: 10.2174/0929866043478329.

DOI:10.2174/0929866043478329

PMID:15078207

Abstract

Alpha-amylase Inhibitors were isolated from Ficus sp. (Gameleira) seeds by acetone fractionation and Sephadex G-50. Two inhibitors (alpha-PPAI and alpha-ZSAI) were tested against alpha-amylases from coleopteran larvae. alpha-PPAI was active to alpha-amylases of Callosobruchus maculatus (52%) and Zabrotes subfasciatus (53%). alpha-ZSAI was strongly active to Z. subfasciatus (100%) of and Mimosestes mimosae (98%). The alpha-ZSAI is a glycoprotein of approximately 50 kDa with an IC50 value of 0.074 microg microl(-1).

摘要

通过丙酮分级分离和葡聚糖凝胶G-50从榕属植物（加梅莱拉）种子中分离出α-淀粉酶抑制剂。测试了两种抑制剂（α-PPAI和α-ZSAI）对鞘翅目幼虫α-淀粉酶的抑制作用。α-PPAI对黄斑豆象（52%）和赤拟谷盗（53%）的α-淀粉酶具有活性。α-ZSAI对赤拟谷盗（100%）和米氏米象（98%）具有强烈活性。α-ZSAI是一种分子量约为50 kDa的糖蛋白，IC50值为0.074 μg μL-1。