热休克蛋白70-同源基因多态性与强直性脊柱炎的关系

[Relationship between heat shock protein 70-hom gene polymorphism and ankylosing spondylitis].

作者信息

Lin Jing-an, Ye De-fu, Zhu Yue-yong, Chen Jun-min, Zheng Wei-qing

机构信息

Center of Research, First Affiliated Hospital, Fujian Medical University, Fuzhou, Fujian, 350005 PR China.

出版信息

Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2004 Apr;21(2):182-3.

PMID:15079808

Abstract

OBJECTIVE

To investigate the association between heat shock protein 70-hom (HSP70-hom) gene polymorphism and ankylosing spondylitis(AS) in Chinese Han patients.

METHODS

Genomic DNA from 98 Chinese AS patients and 70 ethnically matched controls were typed for HSP70-hom polymorphism by polymerase chain reaction-restriction fragment length polymorphism.

RESULTS

The HSP70-hom genotypes in the AS patients consisted of homozygote AA (60.2%) and BB(4.1%), and heterozygote(35.7%), while the HSP70-hom genotypes in the controls were composed of AA(58.6%), BB(2.9%) and heterozygote(38.6%). No significant difference was found in the distribution of HSP70-hom genotype between these two groups(chi(2) test=0.280, P>0.05). The frequencies of HSP70-hom alleles in AS patients were 77.9%(AA) and 22.1%(BB), while they were 78.1% and 21.9% in the controls. The frequency of HSP70-hom allele in AS patients was not significantly increased, compared with that in controls (chi(2) test=0.002, P>0.05).

CONCLUSION

There may be no association between the HSP70-hom gene polymorphism and ankylosing spondylitis in Chinese Han population.

摘要

目的

探讨热休克蛋白70-同源物（HSP70-hom）基因多态性与中国汉族强直性脊柱炎（AS）患者的相关性。

方法

采用聚合酶链反应-限制性片段长度多态性方法，对98例中国AS患者和70例种族匹配的对照者的基因组DNA进行HSP70-hom多态性分型。

结果

AS患者的HSP70-hom基因型由纯合子AA（60.2%）、BB（4.1%）和杂合子（35.7%）组成，而对照组的HSP70-hom基因型由AA（58.6%）、BB（2.9%）和杂合子（38.6%）组成。两组间HSP70-hom基因型分布无显著差异（χ²检验=0.280，P>0.05）。AS患者中HSP70-hom等位基因频率为77.9%（AA）和22.1%（BB），而对照组为78.1%和21.9%。与对照组相比，AS患者中HSP70-hom等位基因频率无显著增加（χ²检验=0.002，P>0.05）。