10 kDa水稻醇溶蛋白种子贮藏蛋白中的氨基酸变异

Amino acid variation in the 10 kDa Oryza prolamin seed storage protein.

作者信息

Mullins Irene M, Hilu Khidir W

机构信息

Department of Health Evaluation Sciences, University of Virginia, P O Box 800717, Charlottesville, Virginia 22908, USA.

出版信息

J Agric Food Chem. 2004 Apr 21;52(8):2242-6. doi: 10.1021/jf0305480.

DOI:10.1021/jf0305480

PMID:15080628

Abstract

The deduced amino acid variability for the 10 kDa prolamin was determined for 16 Oryza species, both cultivated (rice) and wild. Prolamin, a seed storage protein and site of nitrogen and sulfur accumulation, is sequestered in the subaleurone layer of the starchy endosperm for use during seedling germination. The 10 kDa prolamin amino acid distribution for the cultivated species (O. sativa and O. glaberrima) was determined and compared to those of wild and, hitherto unknown, noncultivated Oryza species. Four wild species (O. granulata, O. australiensis, O. brachyantha, and O. meyeriana) exhibited the greatest residue heterogeneity in both the signal and mature peptide regions. A breakdown of the essential amino acid variance among three Central/South American and one African endemic wild species is also presented and compared with those of rice.

摘要

测定了16种栽培（水稻）和野生稻属物种10 kDa醇溶蛋白的推导氨基酸变异性。醇溶蛋白是一种种子贮藏蛋白，也是氮和硫积累的部位，它被隔离在淀粉胚乳的亚糊粉层中，供幼苗萌发时使用。确定了栽培种（亚洲栽培稻和光稃稻）的10 kDa醇溶蛋白氨基酸分布，并与野生稻和迄今未知的非栽培稻属物种进行了比较。四种野生稻（粒形稻、澳洲野生稻、短药野生稻和普通野生稻）在信号肽和成熟肽区域均表现出最大的残基异质性。还给出了三个中南美洲和一个非洲特有野生稻种必需氨基酸变异的分类，并与水稻进行了比较。