Peng H-J, Su S-N, Tsai J-J, Tsai L-C, Kuo H-L, Kuo S-W
Department of Medical Research and Education, Veterans General Hospital-Taipei, Taipei, Taiwan.
Clin Exp Allergy. 2004 Apr;34(4):663-70. doi: 10.1111/j.1365-2222.2004.1925.x.
For genetically predisposed atopic infants, cow's milk protein hydrolysed formulas have been widely used.
Whether hydrolysed formulas can induce oral tolerance to whey proteins will be extensively studied in naïve and sensitized mice.
Antigenicity of hydrolysed formulas was first studied using immunoblotting. Naïve mice fed hydrolysed formulas for 1-4 weeks were sensitized with whey allergens. In contrast, mice sensitized with whey allergens were fed hydrolysed formulas continually for 12 weeks.
Whey allergens were found in Nan and Neoangelac FL. Large whey peptides with antigenicity were found in Nan-HA. Profound suppression of IgE, IgG1 and IgG responses to whey allergens were induced in those fed Nan for 1 week, or Nan-HA for 4 weeks. IgE responses to whey allergens were suppressed in those fed Neoangelac FL for 4 weeks, or Nan-HA for 1-2 weeks. In contrast, those fed extensively hydrolysed formulas for 1-4 weeks failed to show decreased responses. On the other hand, IgE responses to beta-lactoglobulin, but not to bovine serum albumin or alpha-lactalbumin, were decreased in sensitized mice fed Nan for 12 weeks. There was no suppression in sensitized mice fed hydrolysed formulas.
Suppression of IgE responses to whey proteins was readily induced in naïve mice fed Nan or Nan-HA for 1 week. In contrast, it was hardly induced in sensitized mice even after prolonged feeding of Nan for 12 weeks, let alone hydrolysed formulas.
对于具有遗传易感性的特应性婴儿，牛奶蛋白水解配方奶粉已被广泛使用。
将在未致敏和致敏小鼠中广泛研究水解配方奶粉是否能诱导对乳清蛋白的口服耐受。
首先使用免疫印迹法研究水解配方奶粉的抗原性。给未致敏小鼠喂食水解配方奶粉1 - 4周后，用乳清过敏原使其致敏。相比之下，用乳清过敏原致敏的小鼠连续喂食水解配方奶粉12周。
在能恩（Nan）和纽康特安儿宁能恩（Neoangelac FL）中发现了乳清过敏原。在能恩深度水解（Nan - HA）中发现了具有抗原性的大乳清肽。喂食能恩1周或能恩深度水解4周的小鼠中，对乳清过敏原的IgE、IgG1和IgG反应受到显著抑制。喂食纽康特安儿宁能恩4周或能恩深度水解1 - 2周的小鼠中，对乳清过敏原的IgE反应受到抑制。相比之下，喂食广泛水解配方奶粉1 - 4周的小鼠未显示反应降低。另一方面，喂食能恩12周的致敏小鼠中，对β-乳球蛋白的IgE反应降低，但对牛血清白蛋白或α-乳白蛋白的IgE反应未降低。喂食水解配方奶粉的致敏小鼠中没有抑制作用。
喂食能恩或能恩深度水解1周的未致敏小鼠中，对乳清蛋白的IgE反应很容易受到抑制。相比之下，即使致敏小鼠长时间喂食能恩12周，也很难诱导这种抑制作用，更不用说水解配方奶粉了。
