Totani Kiichiro, Matsuo Ichiro, Ito Yukishige
CREST, JST, Kawaguchi 332-1102, Japan.
Bioorg Med Chem Lett. 2004 May 3;14(9):2285-9. doi: 10.1016/j.bmcl.2004.01.106.
A novel type of artificial glycoprotein was developed, by using dihydrofolate reductase (DHFR) and methotrexate (MTX) as a protein-ligand pair. Various oligosaccharides linked to MTX were shown to bind tightly with DHFR and afforded oligosaccharide-grafted protein, which could be isolated easily by lectin beads.
