Dinçer Irem, Ergin Alper, Kocagöz Tanil
Department of Microbiology and Clinical Microbiology, School of Medicine, Hacettepe University, Ankara, Turkey.
Int J Antimicrob Agents. 2004 Apr;23(4):408-11. doi: 10.1016/j.ijantimicag.2003.09.023.
In vitro activity of beta-lactam antibiotics and their beta-lactamase inhibitor combinations were evaluated for their activity against clinical isolates of Mycobacterium tuberculosis and M. tuberculosis H37Ra. Agar dilution, the BACTEC 460 system and beta-lactamase activity tests were used. Results using the BACTEC 460 and enzyme activity tests showed the best beta-lactamase inhibitor for M. tuberculosis H37Ra to be clavulanic acid. Cefazolin-clavulanic acid gave the lowest minimal inhibitory concentration (MIC) values using dilution tests and M. tuberculosis H37Ra. beta-Lactam antibiotics were combined with clavulanic acid and tested for in vitro activity against 50 selected multidrug-resistant (MDR) and 50 susceptible clinical isolates. Seventy-four percent of the isolates were inhibited by cefazolin-clavulanic acid combination. These results suggested that an appropriate combination of beta-lactam and beta-lactamase inhibitors might be useful in the treatment of tuberculosis due to multidrug-resistant strains.
评估了β-内酰胺类抗生素及其与β-内酰胺酶抑制剂组合对结核分枝杆菌临床分离株和结核分枝杆菌H37Ra的体外活性。采用了琼脂稀释法、BACTEC 460系统和β-内酰胺酶活性试验。使用BACTEC 460和酶活性试验的结果表明，对结核分枝杆菌H37Ra而言，最佳的β-内酰胺酶抑制剂是克拉维酸。使用稀释试验对结核分枝杆菌H37Ra进行检测时，头孢唑林-克拉维酸的最低抑菌浓度（MIC）值最低。将β-内酰胺类抗生素与克拉维酸联合，对50株选定的耐多药（MDR）临床分离株和50株敏感临床分离株进行了体外活性检测。74%的分离株被头孢唑林-克拉维酸组合抑制。这些结果表明，β-内酰胺类抗生素与β-内酰胺酶抑制剂的适当组合可能对耐多药菌株所致结核病的治疗有用。
