Laskiewicz J, Królczyk G, Zurowski D, Enck P, Thor P J
Department of Pathophysiology, Jagiellonian University, Cracow, Poland.
J Physiol Pharmacol. 2004 Mar;55(1 Pt 2):155-63.
Study was based on hypothesis that electrical stimulation (ES) with parameters obtained from analysis of vagal afferent discharge fed state may fake brain with satiety state. We evaluated effect of denervation of vagal capsaicin-sensitive afferents on food intake and body weight in rats with ES of vagal nerves using microchip (MC). Group A was scheduled to MC implantation, B to sham operation only, C to MC implantation and capsaicin vagal deafferentation, and D to capsaicin denervation only. ES lasted 24 days. MC parameters were 0.05 Hz, 0.1s, 0.55 V. ES of left vagus significantly reduced total food intake as well as the mean daily intake in groups A and C in comparison to control and D group (ANOVA, F=18.55, p=0.0038). Body weight was lower in group A (3462 g) and C (2727 g) then in control (3814 g) and D (3568 g) (F=25.68, p=0.00068). Leptin decreased in C (165 pg/mL) in comparison to A (625 pg/mL), B (677 pg/mL), and D (612 pg/mL) (p<0,05), mainly due to ES (F=7.27, p=0.019). Glucose was decreased in A (F=5.55, p=0.036) - by 11% and by 16% in C group. Proper vagal neuromodulation results in central and peripheral effects causing food intake and body weight downregulation.
本研究基于这样一种假设，即通过对迷走神经传入放电进食状态分析获得的参数进行电刺激（ES），可能会使大脑误以为处于饱腹感状态。我们使用微芯片（MC）对迷走神经进行电刺激，评估了迷走神经辣椒素敏感传入神经去神经支配对大鼠食物摄入量和体重的影响。A组计划植入MC，B组仅进行假手术，C组进行MC植入和辣椒素迷走神经去传入神经支配，D组仅进行辣椒素去神经支配。电刺激持续24天。MC参数为0.05Hz、0.1秒、0.55V。与对照组和D组相比，左侧迷走神经的电刺激显著降低了A组和C组的总食物摄入量以及平均每日摄入量（方差分析，F = 18.55，p = 0.0038）。A组（3462克）和C组（2727克）的体重低于对照组（3814克）和D组（3568克）（F = 25.68，p = 0.00068）。与A组（625 pg/mL）、B组（677 pg/mL）和D组（612 pg/mL）相比，C组（165 pg/mL）的瘦素水平降低（p < 0.05），主要是由于电刺激（F = 7.27，p = 0.019）。A组的葡萄糖水平降低（F = 5.55，p = 0.036），C组降低了11%和16%。适当的迷走神经神经调节会产生中枢和外周效应，导致食物摄入量和体重下调。
