Singh P, Cobb S, Rengifo-Cam W, Deng X, Willis W, Li Q
Department of Anatomy and Neurosciences, University of Texas Medical Branch, Galveston, Texas 77550-1043, USA.
J Physiol Pharmacol. 2004 Mar;55(1 Pt 2):269-78.
The current studies were initiated to investigate the role of brain-gut peptide, gastrin, on locomotor activity and anxiety-like behavior. Young, male mutant mice, lacking gastrin gene expression (GAS-KO mice), were used in the experiments. The locomotor activity of GAS-KO vs wild type (WT) mice was compared by open field test. The anxiety-like behavior was examined using elevated plus maze. The time and entries to the open arms of the elevated plus maze were used as an indicator for the anxiety-like behavior and the data were analyzed using Hindsight program. On the open field test, locomotor activity of GAS-KO mice was similar to that of the WT mice for the first 10 min of the test, but decreased significantly after that. Anxiety-like behavior was more evident in the GAS-KO vs WT mice in the elevated plus maze experiments. The number of entries to and time spent on the open arms of plus-maze were significantly reduced for the GAS-KO vs WT mice suggesting an increased anxiety-like behavior of GAS-KO mice. Our studies suggest that normal circulating levels of gastrins may play a direct or indirect role in the regulation of locomotor activity and anxiety-like behavior.
开展当前这些研究是为了探究脑肠肽胃泌素在运动活动和焦虑样行为中的作用。实验使用了缺乏胃泌素基因表达的年轻雄性突变小鼠（GAS-KO小鼠）。通过旷场试验比较了GAS-KO小鼠与野生型（WT）小鼠的运动活动。使用高架十字迷宫检测焦虑样行为。将进入高架十字迷宫开放臂的时间和次数用作焦虑样行为的指标，并使用indsight程序分析数据。在旷场试验中，GAS-KO小鼠在试验的前10分钟内的运动活动与WT小鼠相似，但之后显著下降。在高架十字迷宫实验中，GAS-KO小鼠比WT小鼠的焦虑样行为更明显。与WT小鼠相比，GAS-KO小鼠进入十字迷宫开放臂的次数和在开放臂上花费的时间显著减少，表明GAS-KO小鼠的焦虑样行为增加。我们的研究表明，正常循环水平的胃泌素可能在运动活动和焦虑样行为的调节中发挥直接或间接作用。
